Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2012/11/07"

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(Bradford Assay)
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==Objective==
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*Determine concentration of ADA prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] via Bradford Assay
 
==Bradford Assay==
 
==Bradford Assay==
  
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*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed.
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*The ADA was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.
*The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.
 
 
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Revision as of 11:53, 7 November 2012

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Objective

  • Determine concentration of ADA prepared Oct. 23 via Bradford Assay

Bradford Assay

A standard curve was obtained with the following concentrations of BSA:

volume BSA (uL) volume H2O (uL [BSA] (mg/mL) [BSA] (M)
0 30 0 0.00E+00
5 25 0.24 3.61E-06
10 20 0.15 2.26E-06
15 15 0.13 2.01E-06
20 10 0.15 2.26E-06
25 5 0.24 3.61E-06
30 0 1 1.51E-05


  • The ADA was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.