Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2012/11/07"

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(Entry title)
(Bradford Assay)
Line 10: Line 10:
 
A standard curve was obtained with the following concentrations of BSA:
 
A standard curve was obtained with the following concentrations of BSA:
  
[table]
+
{| {{table}}
[tr]
+
| align="center" style="background:#f0f0f0;"|'''volume BSA (uL)'''
[th]volume BSA (uL)[/th]
+
| align="center" style="background:#f0f0f0;"|'''volume H2O (uL'''
[th]volume H2O (uL)[/th]
+
| align="center" style="background:#f0f0f0;"|'''[BSA] (mg/mL)'''
[th]concentration BSA (mg/mL)[/th]
+
| align="center" style="background:#f0f0f0;"|'''[BSA] (M)'''
[th]concentration BSA (molarity)[/th]
+
|-
[/tr]
+
| 0||30||0||0.00E+00
[tr]
+
|-
[td]0[/td]
+
| 5||25||0.24||3.61E-06
[td]30[/td]
+
|-
[td]0[/td]
+
| 10||20||0.15||2.26E-06
[td]0.00E+00[/td]
+
|-
[/tr]
+
| 15||15||0.13||2.01E-06
[tr]
+
|-
[td]5[/td]
+
| 20||10||0.15||2.26E-06
[td]25[/td]
+
|-
[td].24[/td]
+
| 25||5||0.24||3.61E-06
[td]3.61E-06[/td]
+
|-
[/tr]
+
| 30||0||1||1.51E-05
[tr]
+
|-
[td]10[/td]
+
|
[td]20[/td]
+
|}
[td].15[/td]
 
[td]2.26E-06[/td]
 
[/tr]
 
[tr]
 
[td]15[/td]
 
[td]15[/td]
 
[td].13[/td]
 
[td]2.01E-06[/td]
 
[/tr]
 
[tr]
 
[td]20[/td]
 
[td]10[/td]
 
[td].15[/td]
 
[td]2.26E-06[/td]
 
[/tr]
 
[tr]
 
[td]25[/td]
 
[td]5[/td]
 
[td].24[/td]
 
[td]3.61E-06[/td]
 
[/tr]
 
[tr]
 
[td]30[/td]
 
[td]0[/td]
 
[td]1[/td]
 
[td]1.51E-05[/td]
 
[/tr]
 
[/table]
 
 
<Br>
 
<Br>
 
*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed.
 
*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed.

Revision as of 11:51, 7 November 2012

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Bradford Assay

A standard curve was obtained with the following concentrations of BSA:

volume BSA (uL) volume H2O (uL [BSA] (mg/mL) [BSA] (M)
0 30 0 0.00E+00
5 25 0.24 3.61E-06
10 20 0.15 2.26E-06
15 15 0.13 2.01E-06
20 10 0.15 2.26E-06
25 5 0.24 3.61E-06
30 0 1 1.51E-05


  • The ADA made Oct. 23 was analyzed.
  • The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.