User:Michael F. Nagle/Notebook/Chem 571/2012/11/07: Difference between revisions
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==Bradford Assay== | ==Bradford Assay== | ||
* | *Solutions were prepared with 715μL 1x Bio-Rad Bradford reagant and the following concentrations of BSA: | ||
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*A UV/Vis calibration curve was obtained by analyzing the samples and plotting BSA concentration against absorbance at 595nm. | |||
*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06|ADA purified yesterday]] was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration. | *The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/06|ADA purified yesterday]] was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration. | ||
==Data== | |||
[[Image:Bradforassay.png]] | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Fraction #''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance''' | |||
| align="center" style="background:#f0f0f0;"|'''[ADA] [mg/mL]''' | |||
| align="center" style="background:#f0f0f0;"|'''molar absorptivity [mL/mg*cm-1]''' | |||
| align="center" style="background:#f0f0f0;"|'''Molarity (µM)''' | |||
|- | |||
| 2||0.93||0.0177||52.4||42.0 | |||
|- | |||
| 8||0.757||0.0115||65.9||54.4 | |||
|- | |||
| 9||0.819||0.0137||59.7||65.0 | |||
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| | |||
|} | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 04:48, 7 December 2012
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Objective
Bradford Assay
Data
|