Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2012/11/07"

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(Autocreate 2012/11/07 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
 
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==Entry title==
+
==Bradford Assay==
* Insert content here...
 
  
 +
A standard curve was obtained with the following concentrations of BSA:
  
 +
[table]
 +
[tr]
 +
[th]volume BSA (uL)[/th]
 +
[th]volume H2O (uL)[/th]
 +
[th]concentration BSA (mg/mL)[/th]
 +
[th]concentration BSA (molarity)[/th]
 +
[/tr]
 +
[tr]
 +
[td]0[/td]
 +
[td]30[/td]
 +
[td]0[/td]
 +
[td]0.00E+00[/td]
 +
[/tr]
 +
[tr]
 +
[td]5[/td]
 +
[td]25[/td]
 +
[td].24[/td]
 +
[td]3.61E-06[/td]
 +
[/tr]
 +
[tr]
 +
[td]10[/td]
 +
[td]20[/td]
 +
[td].15[/td]
 +
[td]2.26E-06[/td]
 +
[/tr]
 +
[tr]
 +
[td]15[/td]
 +
[td]15[/td]
 +
[td].13[/td]
 +
[td]2.01E-06[/td]
 +
[/tr]
 +
[tr]
 +
[td]20[/td]
 +
[td]10[/td]
 +
[td].15[/td]
 +
[td]2.26E-06[/td]
 +
[/tr]
 +
[tr]
 +
[td]25[/td]
 +
[td]5[/td]
 +
[td].24[/td]
 +
[td]3.61E-06[/td]
 +
[/tr]
 +
[tr]
 +
[td]30[/td]
 +
[td]0[/td]
 +
[td]1[/td]
 +
[td]1.51E-05[/td]
 +
[/tr]
 +
[/table]
 +
<Br>
 +
*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed.
 +
*The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.
 
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Revision as of 10:48, 7 November 2012

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Bradford Assay

A standard curve was obtained with the following concentrations of BSA:

[table] [tr] [th]volume BSA (uL)[/th] [th]volume H2O (uL)[/th] [th]concentration BSA (mg/mL)[/th] [th]concentration BSA (molarity)[/th] [/tr] [tr] [td]0[/td] [td]30[/td] [td]0[/td] [td]0.00E+00[/td] [/tr] [tr] [td]5[/td] [td]25[/td] [td].24[/td] [td]3.61E-06[/td] [/tr] [tr] [td]10[/td] [td]20[/td] [td].15[/td] [td]2.26E-06[/td] [/tr] [tr] [td]15[/td] [td]15[/td] [td].13[/td] [td]2.01E-06[/td] [/tr] [tr] [td]20[/td] [td]10[/td] [td].15[/td] [td]2.26E-06[/td] [/tr] [tr] [td]25[/td] [td]5[/td] [td].24[/td] [td]3.61E-06[/td] [/tr] [tr] [td]30[/td] [td]0[/td] [td]1[/td] [td]1.51E-05[/td] [/tr] [/table]

  • The ADA made Oct. 23 was analyzed.
  • The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.