Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2012/11/06"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(Procedure)
Line 11: Line 11:
  
 
==Procedure==
 
==Procedure==
*The prodedure for [AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation] was followed, with the following modifications
+
*The prodedure for [[AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation]] was followed, with the following modifications
 
**20μL DNA solution was added to 30μL solution with E. Coli cells.
 
**20μL DNA solution was added to 30μL solution with E. Coli cells.
 
**250μL Lysogeny Broth (LB) was used instead of SOC medium.
 
**250μL Lysogeny Broth (LB) was used instead of SOC medium.
 
**Single 200μL plates were prepared. No 50μL plates were made.
 
**Single 200μL plates were prepared. No 50μL plates were made.
*Adenosine Deaminase prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23] was purified via FPLC
+
*Adenosine Deaminase prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was purified via FPLC
 
** Pump flow was 5mL/minute
 
** Pump flow was 5mL/minute
  

Revision as of 11:32, 7 November 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Transform Adenosine Deaminase in cells
  • Purify protein via Fast Protein Liquid Chromatography (FPLC)

Procedure

  • The prodedure for PCR Mutation was followed, with the following modifications
    • 20μL DNA solution was added to 30μL solution with E. Coli cells.
    • 250μL Lysogeny Broth (LB) was used instead of SOC medium.
    • Single 200μL plates were prepared. No 50μL plates were made.
  • Adenosine Deaminase prepared Oct. 23 was purified via FPLC
    • Pump flow was 5mL/minute