User:Michael F. Nagle/Notebook/Chem 571/2012/10/31

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Obtain UV/Vis spectra for Au/Lysozyme samples with varying mole ratios.
  • Purify ADA from E. Coli

Procedure

  • Au/Lysozyme samples prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24|last Wednesday] were analyzed by UV/Vis using disposable plastic cuvettes. The isntrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
  • Samples from 134-140 showed variation in the baseline. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
  • [User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody] purified ADA from E. Coli grown [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]