Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2012/10/31"

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(Autocreate 2012/10/31 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
 
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==Entry title==
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==Objectives==
* Insert content here...
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*Obtain UV/Vis spectra for Au/Lysozyme samples with varying mole ratios.
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*Purify ADA from E. Coli
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==Procedure==
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*Au/Lysozyme samples prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24|last Wednesday] were analyzed by UV/Vis using disposable plastic cuvettes. The isntrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
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*Samples from 134-140 showed variation in the baseline. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
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*[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody] purified ADA from E. Coli grown [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]
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Revision as of 11:12, 31 October 2012

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Objectives

  • Obtain UV/Vis spectra for Au/Lysozyme samples with varying mole ratios.
  • Purify ADA from E. Coli

Procedure

  • Au/Lysozyme samples prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24|last Wednesday] were analyzed by UV/Vis using disposable plastic cuvettes. The isntrument held a blank cuvette of water, which was automatically subtracted from the spectra of the Au/Lysozyme.
  • Samples from 134-140 showed variation in the baseline. In order to determine whether this was due to Lysozyme or variation in cuvettes, these trials were repeated using the same quartz cuvette. A blank of water was run using the same cuvette and subtracted from each spectra manually.
  • [User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/31|Puja Moody] purified ADA from E. Coli grown [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]