User:Michael F. Nagle/Notebook/Chem 571/2012/10/24

From OpenWetWare
< User:Michael F. Nagle‎ | Notebook‎ | Chem 571‎ | 2012‎ | 10
Revision as of 12:12, 24 October 2012 by Michael F. Nagle (talk | contribs) (Entry title)
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  1. 5μL of the DNA solution prepared last week was added to 40μL cells
  2. The solution was placed on a heat block for 30 seconds and put on ice.
  3. The solution was incubated at 37oC and 225rpm for 1 hour
    1. 250μL Lysogeny Broth (LB) was added
  4. The solution was incubated again with the same specifications
  1. Solutions with Lysozyme and BSA prepared [[last week|last week]] were analyzed via UV/Vis. There was no peak at 520nm, so it was determined that heating at 50°C was insufficient. The solutions went back in the oven for 4 hours at 80°C, which is the same temperature BSA was seen succesfully unfolding at.
  2. Solutions with Au and BSA prepared last week were analyzed via UV/Vis.