User:Michael F. Nagle/Notebook/Chem 571/2012/10/16: Difference between revisions
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==Procedure== | ==Procedure== | ||
* The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed | * The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed | ||
** The specifications of the primer used are as follows: | ** The specifications of the forward primer used are as follows: | ||
[[Image:Primer212.png]] | [[Image:Primer212.png]] | ||
**1mL molecular biology grade water was added to the primer. | **1mL molecular biology grade water was added to the primer. | ||
**The volume of primer solution needed to prepare 1mL of 100ng/uL solution was calculated | **The volume of primer solution needed to prepare 1mL of 100ng/uL solution was calculated | ||
***0.49mg/1000μL = 490ng/μL | ***0.49mg/1000μL = 490ng/μL | ||
***100ng/uL*1000uL / 490ng/uL = 204.08μL | ***100ng/uL*1000uL / 490ng/uL = 204.08μL | ||
**[[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/10/16|Keyun Wang]] prepared 100ng/uL solution of the matching forward primer. | |||
**Solution was prepared with the following: | |||
***1uL forward primer ***1uL reverse primer ***1uL wild strand ADA ***5uL 10x Pfu buffer ***40.6uL molecular biology grade water ***1uL Turbo DNA polymerase solution ***0.4Turbo DNA polymerase 25mM dNTPs | |||
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Revision as of 23:45, 6 December 2012
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