Difference between revisions of "User:Michael F. Nagle/Notebook/Chem 571/2012/10/16"

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(Procedure)
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==Procedure==
 
==Procedure==
# The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed  
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* The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed  
# The specifications of the primer used are as follows:
+
** The specifications of the primer used are as follows:
 
+
[[Image:Primer212.png]]
 +
**1mL molecular biology grade water was added to the primer.
 +
**The volume of primer solution needed to prepare 1mL of 100ng/uL solution was calculated
 +
Target concentration: 100ng/uL
 +
***0.49mg/1000μL = 490ng/μL
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***100ng/uL*1000uL / 490ng/uL = 204.08μL
  
 
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Revision as of 22:38, 6 December 2012

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Procedure

  • The procedure for PCR Mutation was followed
    • The specifications of the primer used are as follows:

Primer212.png

    • 1mL molecular biology grade water was added to the primer.
    • The volume of primer solution needed to prepare 1mL of 100ng/uL solution was calculated
Target concentration: 100ng/uL
      • 0.49mg/1000μL = 490ng/μL
      • 100ng/uL*1000uL / 490ng/uL = 204.08μL