User:Michael F. Nagle/Notebook/Chem 571/2012/09/18: Difference between revisions
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#Starter Cultures with 25mL water had .8793g, .8800g, .8748g, and .8757g broth in them. | #Starter Cultures with 25mL water had .8793g, .8800g, .8748g, and .8757g broth in them. | ||
#Expression cultures with 1L water had 25.0289g, 25.0069g, 25.002g, and 25.0036g broth in them. | #Expression cultures with 1L water had 25.0289g, 25.0069g, 25.002g, and 25.0036g broth in them. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 11:25, 7 October 2012 (EDT)''':inidiacte how you distigushedthe flasks..1, 2, 3, 4? or A, B, C, D? how do you know if htey act differently due to the amounts of chemicals added? | |||
#Amount of Kanamycin for cultures was calculated. Kanamycin is an antibiotic and is being used to prevent microbial growth besides that of E. Coli. | #Amount of Kanamycin for cultures was calculated. Kanamycin is an antibiotic and is being used to prevent microbial growth besides that of E. Coli. | ||
##The molarity was converted to µg/mL and the equation M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> was used to calculate the volume of stock that should go in broth. | ##The molarity was converted to µg/mL and the equation M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> was used to calculate the volume of stock that should go in broth. | ||
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#The foil caps on the broth flasks was sterilized with a bunsen burner after each time they were removed and placed back on the flasks. Metal forceps, which were also sterilized with flame between uses, were used to pick up a splint, which was used to pick up one colony of E. Coli and transfer it to the broth. A different split was used each time. | #The foil caps on the broth flasks was sterilized with a bunsen burner after each time they were removed and placed back on the flasks. Metal forceps, which were also sterilized with flame between uses, were used to pick up a splint, which was used to pick up one colony of E. Coli and transfer it to the broth. A different split was used each time. | ||
#The plasmid that codes for Adenosine Deaminase (ADA)was inserted into the broth. | #The plasmid that codes for Adenosine Deaminase (ADA)was inserted into the broth. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 11:25, 7 October 2012 (EDT)''':plasmid is in the e. coli, therefore you add a colony of E. coli cells that contain the plasmid for ADA. split? | |||
#The flasks were placed in an orbital incubator shaker at 37°C and 237 rpm | #The flasks were placed in an orbital incubator shaker at 37°C and 237 rpm | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 11:25, 7 October 2012 (EDT)''':not that this was left to shake overnight | |||
==Discussion== | ==Discussion== |
Revision as of 08:25, 7 October 2012
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Objectives
ProcedureStarter Culture and Expression Culture
Discussion
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