Difference between revisions of "User:Meng Xiao He/Notebook/fall08/2008/12/15"

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(PCR retry)
(PCR retry)
 
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[[Image:2008-12-15_pcr_plate.jpg]]
 
[[Image:2008-12-15_pcr_plate.jpg]]
  
 +
However, it seems that the ones with bands are the only PCRs that remotely worked; the bands are actually the upper bands:
 +
[[Image:12-16pcr_redo.jpg]]
  
 +
Will have to troubleshoot this PCR...
 
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Latest revision as of 10:27, 16 December 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page<html>      </html> Constants
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PCR retry

  • retried PCR using 15ul volume and all 3 outer primer sets, 12-8 conditions
  • note that 24 lane means sets of 12 are staggered:

2008-12-15 pcr plate.jpg

However, it seems that the ones with bands are the only PCRs that remotely worked; the bands are actually the upper bands: 12-16pcr redo.jpg

Will have to troubleshoot this PCR...