Difference between revisions of "User:Meng Xiao He/Notebook/fall08/2008/12/13"

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< User:Meng Xiao He‎ | Notebook‎ | fall08‎ | 2008‎ | 12
(Title)
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[[Image:12-13pcr.jpg]]
 
[[Image:12-13pcr.jpg]]
 
*PCR seems to have failed even more than usual
 
*PCR seems to have failed even more than usual
 +
** cco colonies 1 and 5 are definitely WT (either revertants or previous PCR just didn't amplify the band properly)
 
*new PCRs set up with colonies that are sucroseR but not GmR
 
*new PCRs set up with colonies that are sucroseR but not GmR
 
** 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things...
 
** 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things...
 
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Revision as of 10:04, 13 December 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html> Constants
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First 6 are cco, next 5 are cbb

12-13pcr.jpg

  • PCR seems to have failed even more than usual
    • cco colonies 1 and 5 are definitely WT (either revertants or previous PCR just didn't amplify the band properly)
  • new PCRs set up with colonies that are sucroseR but not GmR
    • 20ul reaction, revert back to 12-8 in case longer extension time is doing bad things...