Difference between revisions of "User:Meng Xiao He/Notebook/fall08/2008/12/12"

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(Replating on Gm, sucrose)
(Replating on Gm, sucrose)
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==Replating on Gm, sucrose==
 
==Replating on Gm, sucrose==
 
* colonies replated on both sucrose and Gm
 
* colonies replated on both sucrose and Gm
* 5 cbb and 6 cco colonies Phusion PCRed using PhFLANK program (12-8 modified to have 3:45 extension) > note that flanking pairs were only the ko gene and Duo as the control (no orientation primer)
+
* 5 cbb and 6 cco colonies Phusion PCRed using PhFLANK program (12-8 modified to have 3:45 extension) > flanking primer pairs were only the ko gene and Duo as the control (no orientation primer)
  
 
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Revision as of 10:30, 12 December 2008

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Replating on Gm, sucrose

  • colonies replated on both sucrose and Gm
  • 5 cbb and 6 cco colonies Phusion PCRed using PhFLANK program (12-8 modified to have 3:45 extension) > flanking primer pairs were only the ko gene and Duo as the control (no orientation primer)