User:Melissa Novy/Notebook/CHEM-581/2012/11/07

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Objectives

  • Analyze solutions of porphine and either Al2(SO4)3, Fe2(SO4)3 FeSO4, or Na2SO4 in HPLC H2O.

Absorbance of Solutions of 1.89 μM Porphine and Varied Concentration of Salt in HPLC H2O

  • The concentrations of salt in a total solution volume of 10 mL, with porphine concentration of 1.89 μM were as follows:
    • 1 mM, 2 mM, 5 mM, and 10 mM Al2(SO4)3
    • 1 mM, 2 mM, 5 mM, and 10 mM Fe2(SO4)3
    • 1 mM, 2 mM, 5 mM, and 10 mM FeSO4
    • 1 mM, 2 mM, 5 mM, and 10 mM Na2SO4
  • There were 16 solutions in total.

Volumes and Concentrations of Porphine and Salt in Solution

  • Initial Concentrations of Stock Solutions
Porphine Al2(SO4)3 Fe2(SO4)3 FeSO4 Na2SO4
9.445 uM 10 mM 10 mM 10 mM 10 mM


  • Volumes of Stock Solutions Added to Make Sample Solutions
Porphine Al2(SO4)3 Fe2(SO4)3 FeSO4 Na2SO4
2 0.1 0.1 0.1 0.1
2 0.2 0.2 0.2 0.2
2 0.5 0.5 0.5 0.5
2 1 1 1 1


  • Final Concentrations of Porphine and Salt in Sample Solutions
Porphine Al2(SO4)3 Fe2(SO4)3 FeSO4 Na2SO4 Volume of Water Added [mL]
1.89 µM 1 mM 1 mM 1 mM 1 mM 9.9
1.89 µM 2 mM 2 mM 2 mM 2 mM 9.8
1.89 µM 5 mM 5 mM 5 mM 5 mM 9.5
1.89 µM 10 mM 10 mM 10 mM 10 mM 9

UV-Vis Spectra of 1.89 μM Porphine and Salt Solutions

Al2(SO4)3.jpg

Fe2(SO4)3.jpg

FeSO4.jpg

Na2SO4.jpg