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(Absorbance of Solutions of 1.89 μM Porphine and Varied Concentration of Salt in HPLC H2O)
(Absorbance of Solutions of 1.89 μM Porphine and Varied Concentration of Salt in HPLC H2O)
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Volumes and Concentrations of Porphine and Salt in Solution
 
Volumes and Concentrations of Porphine and Salt in Solution
 +
* Initial Concentrations of Stock Solutions
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Porphine'''
 +
| align="center" style="background:#f0f0f0;"|'''Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''FeSO<sub>4</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>SO<sub>4</sub>'''
 +
|-
 +
| 9.445 uM||10 mM||10 mM||10 mM||10 mM
 +
|}
 +
<br>
 +
* Volumes of Stock Solutions Added to Make Sample Solutions
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Porphine'''
 +
| align="center" style="background:#f0f0f0;"|'''Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''FeSO<sub>4</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>SO<sub>4</sub>'''
 +
|-
 +
| 2||0.1||0.1||0.1||0.1
 +
|-
 +
| 2||0.2||0.2||0.2||0.2
 +
|-
 +
| 2||0.5||0.5||0.5||0.5
 +
|-
 +
| 2||1||1||1||1
 +
|}
 +
<br>
 +
* Final Concentrations of Porphine and Salt in Sample Solutions
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Porphine'''
 +
| align="center" style="background:#f0f0f0;"|'''Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''FeSO<sub>4</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>SO<sub>4</sub>'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume of Water Added [mL]'''
 +
|-
 +
| 1.89 µM||1 mM||1 mM||1 mM||1 mM||9.9
 +
|-
 +
| 1.89 µM||2 mM||2 mM||2 mM||2 mM||9.8
 +
|-
 +
| 1.89 µM||5 mM||5 mM||5 mM||5 mM||9.5
 +
|-
 +
| 1.89 µM||10 mM||10 mM||10 mM||10 mM||9
 +
|}
  
 
+
==UV-Vis Spectra of 1.89 μM Porphine and Salt Solutions==
 
[[Image:Al2%28SO4%293.jpg]]
 
[[Image:Al2%28SO4%293.jpg]]
 
<br><br>
 
<br><br>
Line 36: Line 81:
 
<br><br>
 
<br><br>
 
[[Image:Na2SO4.jpg]]
 
[[Image:Na2SO4.jpg]]
 
  
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
 
  
 
__NOTOC__
 
__NOTOC__

Revision as of 17:04, 8 November 2012

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Objectives

  • Analyze solutions of porphine and either Al2(SO4)3, Fe2(SO4)3 FeSO4, or Na2SO4 in HPLC H2O.

Absorbance of Solutions of 1.89 μM Porphine and Varied Concentration of Salt in HPLC H2O

  • The concentrations of salt in a total solution volume of 10 mL, with porphine concentration of 1.89 μM were as follows:
    • 1 mM, 2 mM, 5 mM, and 10 mM Al2(SO4)3
    • 1 mM, 2 mM, 5 mM, and 10 mM Fe2(SO4)3
    • 1 mM, 2 mM, 5 mM, and 10 mM FeSO4
    • 1 mM, 2 mM, 5 mM, and 10 mM Na2SO4
  • There were 16 solutions in total.

Volumes and Concentrations of Porphine and Salt in Solution

  • Initial Concentrations of Stock Solutions
Porphine Al2(SO4)3 Fe2(SO4)3 FeSO4 Na2SO4
9.445 uM 10 mM 10 mM 10 mM 10 mM


  • Volumes of Stock Solutions Added to Make Sample Solutions
Porphine Al2(SO4)3 Fe2(SO4)3 FeSO4 Na2SO4
2 0.1 0.1 0.1 0.1
2 0.2 0.2 0.2 0.2
2 0.5 0.5 0.5 0.5
2 1 1 1 1


  • Final Concentrations of Porphine and Salt in Sample Solutions
Porphine Al2(SO4)3 Fe2(SO4)3 FeSO4 Na2SO4 Volume of Water Added [mL]
1.89 µM 1 mM 1 mM 1 mM 1 mM 9.9
1.89 µM 2 mM 2 mM 2 mM 2 mM 9.8
1.89 µM 5 mM 5 mM 5 mM 5 mM 9.5
1.89 µM 10 mM 10 mM 10 mM 10 mM 9

UV-Vis Spectra of 1.89 μM Porphine and Salt Solutions

Al2(SO4)3.jpg

Fe2(SO4)3.jpg

FeSO4.jpg

Na2SO4.jpg