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==Objectives==
 
==Objectives==
 
* Crosslink Na-MT films.
 
* Crosslink Na-MT films.
 +
** Please refer to [[User:Moira_M._Esson/Notebook/CHEM-581/2012/10/03|Moira Esson's entry]] for all calculations and observations.
 +
** The following films were crosslinked: PVOH (MW 22000), 0.8 wt% glutaraldehyde, 0.1 wt% 100% CEC NaMT; PVOH (MW 22000), 0.8 wt% glutaraldehyde, 0.1 wt% NaMT; PVOH (MW 22000), 0.8 wt% glutaraldehyde, PDMS.
 
* Adjust the pH of CuSO<sub>4</sub> solutions made on [[User:Melissa_Novy/Notebook/CHEM-581/2012/09/28|2012/09/28]], then take ISE measurements.
 
* Adjust the pH of CuSO<sub>4</sub> solutions made on [[User:Melissa_Novy/Notebook/CHEM-581/2012/09/28|2012/09/28]], then take ISE measurements.
  
==CuSO<sub>4</sub> Standard and Filtrate Solutions==
+
==CuSO<sub>4</sub> Standard, Na<sup>+</sup> Standard, and Na-MT Filtrate Solutions==
 
* Please refer to the ISE protocol on [[User:Melissa_Novy/Notebook/CHEM-581/2012/09/28|2012/09/28]] for how ISE measurements were taken.
 
* Please refer to the ISE protocol on [[User:Melissa_Novy/Notebook/CHEM-581/2012/09/28|2012/09/28]] for how ISE measurements were taken.
 
** 3 mL of the solutions were added to 0.1 mL of ionic strength adjuster (4 M solution containing NaOH) to adjust the pH to a value between 8 and 10.
 
** 3 mL of the solutions were added to 0.1 mL of ionic strength adjuster (4 M solution containing NaOH) to adjust the pH to a value between 8 and 10.
 
** The solutions were allowed to equilibrate with the electrode for 2 min.
 
** The solutions were allowed to equilibrate with the electrode for 2 min.
 +
* Dilutions of 5 mL each of 1, 5, 10, 15, and 20 ppm Na<sup>+</sup> in pure H<sub>2</sub>O were made from a 1000 ppm Na<sup>+</sup> solution, then ISE measurements were taken.
 +
* Calculations
 +
  '''5 mL 20 ppm Na<sup>+</sup> requires:'''
 +
  '''(1000 ppm Na<sup>+</sup>)V<sub>1</sub>=(20 ppm Na<sup>+</sup>)(5 mL)'''
 +
  '''V<sub>1</sub>=100 μL'''
 +
<br>
 +
  '''5 mL 10 ppm Na<sup>+</sup> requires:'''
 +
  '''(1000 ppm Na<sup>+</sup>)V<sub>1</sub>=(10 ppm Na<sup>+</sup>)(5 mL)'''
 +
  '''V<sub>1</sub>=50 μL'''
 +
<br>
 +
  '''5 mL 5 ppm Na<sup>+</sup> requires:'''
 +
  '''(1000 ppm Na<sup>+</sup>)V<sub>1</sub>=(5 ppm Na<sup>+</sup>)(5 mL)'''
 +
  '''V<sub>1</sub>=25 μL'''
 +
<br>
 +
  '''5 mL 5 ppm Na<sup>+</sup> requires:'''
 +
  '''(1000 ppm Na<sup>+</sup>)V<sub>1</sub>=(5 ppm Na<sup>+</sup>)(5 mL)'''
 +
  '''V<sub>1</sub>=25 μL'''
 +
<br>
 +
  '''5 mL 1 ppm Na<sup>+</sup> requires:'''
 +
  '''(1000 ppm Na<sup>+</sup>)V<sub>1</sub>=(1 ppm Na<sup>+</sup>)(5 mL)'''
 +
  '''V<sub>1</sub>=5 μL'''
 
<br>
 
<br>
 
CuSO<sub>4</sub> Standard Solutions
 
CuSO<sub>4</sub> Standard Solutions
Line 38: Line 61:
 
|-
 
|-
 
| 15||9.45||-151.1
 
| 15||9.45||-151.1
 +
|}
 +
<br>
 +
[[Image:CuSO4_ISE_Standards.JPG]]
 +
<br><br>
 +
Na<sup>+</sup> Standard Solutions
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration [ppm]'''
 +
| align="center" style="background:#f0f0f0;"|'''ISE [mV]'''
 +
|-
 +
| 1||-147
 +
|-
 +
| 5||-158
 +
|-
 +
| 10||-145.5
 +
|-
 +
| 20||-132.3
 
|}
 
|}
 
<br>
 
<br>
 
100% CEC (Na-MT)-CuSO<sub>4</sub> Filtrate
 
100% CEC (Na-MT)-CuSO<sub>4</sub> Filtrate
 
{| {{table}}
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cu+2 Concentration [ppm]'''
+
| align="center" style="background:#f0f0f0;"|'''Cu<sup>+2</sup> Concentration [ppm]'''
 
| align="center" style="background:#f0f0f0;"|'''Time [min]'''
 
| align="center" style="background:#f0f0f0;"|'''Time [min]'''
 
| align="center" style="background:#f0f0f0;"|'''pH'''
 
| align="center" style="background:#f0f0f0;"|'''pH'''
Line 61: Line 100:
 
<br>
 
<br>
 
50% CEC (Na-MT)-CuSO<sub>4</sub> Filtrate
 
50% CEC (Na-MT)-CuSO<sub>4</sub> Filtrate
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Cu<sup>+2</sup> Concentration [ppm]'''
 +
| align="center" style="background:#f0f0f0;"|'''Time [min]'''
 +
| align="center" style="background:#f0f0f0;"|'''ISE [mV]'''
 +
|-
 +
| 7.4||1||-172.7
 +
|-
 +
| 10||1||-171.4
 +
|-
 +
| 7.4||5||-174.8
 +
|-
 +
| 10||5||-174.2
 +
|-
 +
| 7.4||10||-168.9
 +
|-
 +
| 10||10||-172.8
 +
|}
 +
<br>
 +
Unmodified (Na-MT)-CuSO<sub>4</sub> Filtrate
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Cu<sup>+2</sup> Concentration [ppm]'''
 +
| align="center" style="background:#f0f0f0;"|'''Time [min]'''
 +
| align="center" style="background:#f0f0f0;"|'''pH'''
 +
| align="center" style="background:#f0f0f0;"|'''ISE [mV]'''
 +
|-
 +
| 7.4||1||8.37||-128.2
 +
|-
 +
| 10||1||8.45||-142.1
 +
|-
 +
| 7.4||5||8.47||-112.7
 +
|-
 +
| 10||5||8.5||-99.2
 +
|-
 +
| 7.4||10||8.44||-138
 +
|-
 +
| 10||10||8.44||-134.5
 +
|}
  
 
+
==Notes==
 
+
* The 1 ppm Na<sup>+</sup> solution gave an ISE value that did not fit the expected calibration curve.  This may be because the detection limit of the ISE measurements is above 1 ppm.  It was recommended that a solution of higher Na<sup>+</sup> ppm, such as 25 ppm Na<sup>+</sup> be tested to generate a more accurate calibration curve.
 +
* The ISE values of the CuSO<sub>4</sub> solutions are meaningless as they were obtained with a sodium electrode.
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
  
 
__NOTOC__
 
__NOTOC__

Revision as of 09:22, 22 October 2012

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Objectives

  • Crosslink Na-MT films.
    • Please refer to Moira Esson's entry for all calculations and observations.
    • The following films were crosslinked: PVOH (MW 22000), 0.8 wt% glutaraldehyde, 0.1 wt% 100% CEC NaMT; PVOH (MW 22000), 0.8 wt% glutaraldehyde, 0.1 wt% NaMT; PVOH (MW 22000), 0.8 wt% glutaraldehyde, PDMS.
  • Adjust the pH of CuSO4 solutions made on 2012/09/28, then take ISE measurements.

CuSO4 Standard, Na+ Standard, and Na-MT Filtrate Solutions

  • Please refer to the ISE protocol on 2012/09/28 for how ISE measurements were taken.
    • 3 mL of the solutions were added to 0.1 mL of ionic strength adjuster (4 M solution containing NaOH) to adjust the pH to a value between 8 and 10.
    • The solutions were allowed to equilibrate with the electrode for 2 min.
  • Dilutions of 5 mL each of 1, 5, 10, 15, and 20 ppm Na+ in pure H2O were made from a 1000 ppm Na+ solution, then ISE measurements were taken.
  • Calculations
  5 mL 20 ppm Na+ requires:
  (1000 ppm Na+)V1=(20 ppm Na+)(5 mL)
  V1=100 μL


  5 mL 10 ppm Na+ requires:
  (1000 ppm Na+)V1=(10 ppm Na+)(5 mL)
  V1=50 μL


  5 mL 5 ppm Na+ requires:
  (1000 ppm Na+)V1=(5 ppm Na+)(5 mL)
  V1=25 μL


  5 mL 5 ppm Na+ requires:
  (1000 ppm Na+)V1=(5 ppm Na+)(5 mL)
  V1=25 μL


  5 mL 1 ppm Na+ requires:
  (1000 ppm Na+)V1=(1 ppm Na+)(5 mL)
  V1=5 μL


CuSO4 Standard Solutions

Concentration [ppm] pH ISE [mV]
1 9.52 -215.5
2 9.48 -207.5
5 9.47 -207.9
10 9.43 -211.7
15 9.45 -151.1


CuSO4 ISE Standards.JPG

Na+ Standard Solutions

Concentration [ppm] ISE [mV]
1 -147
5 -158
10 -145.5
20 -132.3


100% CEC (Na-MT)-CuSO4 Filtrate

Cu+2 Concentration [ppm] Time [min] pH ISE [mV]
7.4 1 9.53 -201.2
10 1 8.47 -174.1
7.4 5 9.52 -200.5
10 5 8.45 -172.5
7.4 10 8.51 -172.3
10 10 9.53 -199


50% CEC (Na-MT)-CuSO4 Filtrate

Cu+2 Concentration [ppm] Time [min] ISE [mV]
7.4 1 -172.7
10 1 -171.4
7.4 5 -174.8
10 5 -174.2
7.4 10 -168.9
10 10 -172.8


Unmodified (Na-MT)-CuSO4 Filtrate

Cu+2 Concentration [ppm] Time [min] pH ISE [mV]
7.4 1 8.37 -128.2
10 1 8.45 -142.1
7.4 5 8.47 -112.7
10 5 8.5 -99.2
7.4 10 8.44 -138
10 10 8.44 -134.5

Notes

  • The 1 ppm Na+ solution gave an ISE value that did not fit the expected calibration curve. This may be because the detection limit of the ISE measurements is above 1 ppm. It was recommended that a solution of higher Na+ ppm, such as 25 ppm Na+ be tested to generate a more accurate calibration curve.
  • The ISE values of the CuSO4 solutions are meaningless as they were obtained with a sodium electrode.