Difference between revisions of "User:Melissa Novy/Notebook/CHEM-572/2013/03/05"

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==Entry title==
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==Objectives==
* Insert content here...
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* Make 16 separate 50-mL portions of LB media in 250 mL Erlenmeyer flasks.
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* Autoclave the 16 solutions of LB media and three boxes of 1000 μL pipet tips.
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* Make a 3-gram film of PLA2002D + 10 wt% 100Ag-LMT.
 +
 
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==LB Media==
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* Sixteen 250-mL Erlenmeyer flasks were washed and dried.
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* Then, 1.25 g of LB media, obtained from Teknova, and 50 mL of deionized water was added to each flask.
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* The flasks were capped with foil and autoclaved, according to the usual [[AU_Biomaterials_Design_Lab:Protocols/Autoclave|procedure]].
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==PLA2002D + 10 wt% 100Ag-LMT Film==
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* Theoretical mass PLA2002D: 3.000 g
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* Theoretical mass 100Ag-LMT: 0.30 g
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<br>
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* Actual mass PLA2002D: 3.00600 g
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* Actual mass 100Ag-LMT: 0.30430 g
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<br>
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* 100Ag-LMT made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/05|2013/02/05]] was used.
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* Please refer to the entry on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/06|2013/02/06]] for the protocol used to make the films.
  
  

Latest revision as of 18:10, 23 September 2017

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Objectives

  • Make 16 separate 50-mL portions of LB media in 250 mL Erlenmeyer flasks.
  • Autoclave the 16 solutions of LB media and three boxes of 1000 μL pipet tips.
  • Make a 3-gram film of PLA2002D + 10 wt% 100Ag-LMT.

LB Media

  • Sixteen 250-mL Erlenmeyer flasks were washed and dried.
  • Then, 1.25 g of LB media, obtained from Teknova, and 50 mL of deionized water was added to each flask.
  • The flasks were capped with foil and autoclaved, according to the usual procedure.

PLA2002D + 10 wt% 100Ag-LMT Film

  • Theoretical mass PLA2002D: 3.000 g
  • Theoretical mass 100Ag-LMT: 0.30 g


  • Actual mass PLA2002D: 3.00600 g
  • Actual mass 100Ag-LMT: 0.30430 g


  • 100Ag-LMT made on 2013/02/05 was used.
  • Please refer to the entry on 2013/02/06 for the protocol used to make the films.