Difference between revisions of "User:Melissa Novy/Notebook/CHEM-572/2013/02/20"

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(Objectives)
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* Calculate the amount of AgNO<sub>3</sub> to add to LB media solutions for desired final [Ag<sup>+</sup>].
 
* Calculate the amount of AgNO<sub>3</sub> to add to LB media solutions for desired final [Ag<sup>+</sup>].
 
* Incubate the silver-doped LB media solutions for 15 hours to monitor bacterial growth.
 
* Incubate the silver-doped LB media solutions for 15 hours to monitor bacterial growth.
 +
 +
==Calculations==
 +
* Two sample calculations are given as examples.  The final [Ag<sup>+</sup>] in 50-mL solutions of LB media was calculated as follows:
 +
  '''1 μM Ag<sup>+</sup> in 50 mL of LB media'''
 +
  '''(0.0105 M Ag<sup>+</sup>) × V<sub>1</sub> = (1×10<sup>-6</sup> M) × (50 mL)'''
 +
  '''V<sub>1</sub> = 0.00476 mL of 0.0105 M AgNO<sub>3</sub>'''
 +
 +
  '''10 μM Ag<sup>+</sup> in 50 mL of LB media'''
 +
  '''(0.1 M Ag<sup>+</sup>) × V<sub>1</sub> = (10×10<sup>-6</sup> M) × (50 mL)'''
 +
  '''V<sub>1</sub> = 0.005 mL of 0.1 M AgNO<sub>3</sub>'''
 +
 +
* AgNO<sub>3</sub> solutions made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/05|2013/02/05]] and [[User:Melissa_Novy/Notebook/CHEM-572/2013/02/13|2013/02/13]] were added to the LB media solutions in the following amounts:
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Final [Ag<sup>+</sup>]'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume of AgNO<sub>3</sub> Solution Added'''
 +
|-
 +
| 1 μM||4.76 μL of 0.0105 M
 +
|-
 +
| 10 μM||5.00 μL of 0.1 M
 +
|-
 +
| 100 μM||50.0 μL of 0.1 M
 +
|-
 +
| 1 mM||500 μL of 0.1 M
 +
|-
 +
| 10 mM||5000 μL of 0.1 M
 +
|}
 +
 +
* Note that for each volume of AgNO<sub>3</sub> added to the LB media, the same volume of LB media was first removed from the flask to maintain the final concentration of Ag<sup>+</sup>.
  
  
 
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|}
 
  
 
__NOTOC__
 
__NOTOC__

Revision as of 12:23, 19 February 2013

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Objectives

  • Calculate the amount of AgNO3 to add to LB media solutions for desired final [Ag+].
  • Incubate the silver-doped LB media solutions for 15 hours to monitor bacterial growth.

Calculations

  • Two sample calculations are given as examples. The final [Ag+] in 50-mL solutions of LB media was calculated as follows:
  1 μM Ag+ in 50 mL of LB media
  (0.0105 M Ag+) × V1 = (1×10-6 M) × (50 mL)
  V1 = 0.00476 mL of 0.0105 M AgNO3
  10 μM Ag+ in 50 mL of LB media
  (0.1 M Ag+) × V1 = (10×10-6 M) × (50 mL)
  V1 = 0.005 mL of 0.1 M AgNO3
  • AgNO3 solutions made on 2013/02/05 and 2013/02/13 were added to the LB media solutions in the following amounts:
Final [Ag+] Volume of AgNO3 Solution Added
1 μM 4.76 μL of 0.0105 M
10 μM 5.00 μL of 0.1 M
100 μM 50.0 μL of 0.1 M
1 mM 500 μL of 0.1 M
10 mM 5000 μL of 0.1 M
  • Note that for each volume of AgNO3 added to the LB media, the same volume of LB media was first removed from the flask to maintain the final concentration of Ag+.