Difference between revisions of "User:Melissa Novy/Notebook/CHEM-572/2013/01/30"

From OpenWetWare
< User:Melissa Novy‎ | Notebook‎ | CHEM-572‎ | 2013‎ | 01
Jump to: navigation, search
(Autocreate 2013/01/30 Entry for User:Melissa_Novy/Notebook/CHEM-572)
 
(fix raw html help text)
 
(3 intermediate revisions by one other user not shown)
Line 10: Line 10:
 
<!-- ## END search column  ## -->
 
<!-- ## END search column  ## -->
 
|-  
 
|-  
|colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
+
|colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Image:Help.png|frameless|link=Help:Notebook/Project_Base/Customize_entry_page]]
 
|-  
 
|-  
 
|colspan="2"|
 
|colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
  
==Entry title==
+
==Objectives==
* Insert content here...
+
* Make 6 sets of 50-mL LB media.
 +
* Make 500 mL agar solution.
 +
* Autoclave LB media and agar solution.
 +
* Make 20 agar plates.
 +
* Filter and dry LMT-95Ag, made on [[User:Melissa_Novy/Notebook/CHEM-572/2013/01/29|2013/01/29]].
  
 +
==LB Media==
 +
* Protocol
 +
*# Obtain 6 clean, dry 250-mL Erlenmeyer flasks.
 +
*# Place 1.25 g of LB medium, obtained from Teknova, into each flask.
 +
*# Fill each flask with 50 mL deionized H<sub>2</sub>O.
 +
*# Cap each flask with aluminum foil and swirl until the LB dissolves.
 +
*# Autoclave the flasks according to the [[AU_Biomaterials_Design_Lab:Protocols/Autoclave|AU Biomaterials Design Lab protocol]].
 +
 +
* Note that the protocol was adapted from the [[LB|OpenWetWare protocol]].
 +
 +
==Agar Solution and Agar Plates==
 +
* Protocol
 +
*# Obtain a clean, dry 1000-mL Erlenmeyer flask.
 +
*# Place 12.5 g of LB medium, obtained from Teknova, into the flask.
 +
*# Fill the flask with 500 mL deionized H<sub>2</sub>O.
 +
*# Swirl the flask to dissolve the LB, then add 7.5 g Trypticase Soy Agar, obtained from Aldrich.
 +
*# Cap the flask with aluminum foil and autoclave according to the [[AU_Biomaterials_Design_Lab:Protocols/Autoclave|AU Biomaterials Design Lab protocol]].
 +
*# Obtain 20 sterile 10-cm Petri dishes.
 +
*# Fill each Petri dish with 25 mL of autoclaved agar solution.
 +
 +
==Filter and Dry LMT-95Ag==
 +
* LMT-95Ag was covered with aluminum foil and left to stir for 24 h.  It was then filtered with Whatman 41 filter paper and a clean, dry filter flask and funnel.  It was washed with 10 mL of (50:50) H<sub>2</sub>O-EtOH.
 +
* LMT-95Ag was then scraped from the filter paper with a spatula and placed in an aluminum dish loosely capped with foil in an 80°C oven to dry overnight.
 +
* The filtrate was reserved for further testing for sodium.
 +
 +
* Observations
 +
** LMT-95Ag turned from opaque white in color to opaque dark gray.  This indicates possible oxidation of Ag.
 +
** The filtrate was clear and colorless, but became transparent red-gray in color after about 30 min.  The filtrate was subsequently covered in foil.
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 17:56, 23 September 2017

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">02/05/2013</div><div style="display:none;" id="page">User:Melissa Novy/Notebook/CHEM-572/2013/01/30</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages Help.png

Objectives

  • Make 6 sets of 50-mL LB media.
  • Make 500 mL agar solution.
  • Autoclave LB media and agar solution.
  • Make 20 agar plates.
  • Filter and dry LMT-95Ag, made on 2013/01/29.

LB Media

  • Protocol
    1. Obtain 6 clean, dry 250-mL Erlenmeyer flasks.
    2. Place 1.25 g of LB medium, obtained from Teknova, into each flask.
    3. Fill each flask with 50 mL deionized H2O.
    4. Cap each flask with aluminum foil and swirl until the LB dissolves.
    5. Autoclave the flasks according to the AU Biomaterials Design Lab protocol.

Agar Solution and Agar Plates

  • Protocol
    1. Obtain a clean, dry 1000-mL Erlenmeyer flask.
    2. Place 12.5 g of LB medium, obtained from Teknova, into the flask.
    3. Fill the flask with 500 mL deionized H2O.
    4. Swirl the flask to dissolve the LB, then add 7.5 g Trypticase Soy Agar, obtained from Aldrich.
    5. Cap the flask with aluminum foil and autoclave according to the AU Biomaterials Design Lab protocol.
    6. Obtain 20 sterile 10-cm Petri dishes.
    7. Fill each Petri dish with 25 mL of autoclaved agar solution.

Filter and Dry LMT-95Ag

  • LMT-95Ag was covered with aluminum foil and left to stir for 24 h. It was then filtered with Whatman 41 filter paper and a clean, dry filter flask and funnel. It was washed with 10 mL of (50:50) H2O-EtOH.
  • LMT-95Ag was then scraped from the filter paper with a spatula and placed in an aluminum dish loosely capped with foil in an 80°C oven to dry overnight.
  • The filtrate was reserved for further testing for sodium.
  • Observations
    • LMT-95Ag turned from opaque white in color to opaque dark gray. This indicates possible oxidation of Ag.
    • The filtrate was clear and colorless, but became transparent red-gray in color after about 30 min. The filtrate was subsequently covered in foil.