# User:Melissa Novy/Notebook/CHEM-571/2012/11/14

< User:Melissa Novy‎ | Notebook‎ | CHEM-571‎ | 2012‎ | 11

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## Objectives

• Make Au-HRP solutions at mole ratios of Au:HRP from 60 to 150.
• Analyze the concentration of HRP stock solution with UV-vis.
• The molar absorption coefficient of HRP at 403 nm is 1.02×105 M-1 cm-1.

## Au-HRP Solutions

• Ten solutions at Au-HRP mole ratios of 60, 70, 80, 90, 100, 110, 120, 130, 140, and 150 were made.
• Please refer to Dhea Patel's entry for theoretical and actual calculations of stock solutions, and volumes of stock solutions and deionized H2O added to each solution.
• Note that 130, 140, 150 Au-HRP solutions were recalculated to account for a new stock solution at different concentration of HRP.
• The concentration of the new HRP stock solution was calculated from its absorbance at 403 nm and its molar absorptivity coefficient.

```  Initial concentration of ADA: 10.35 μM in 1 mL of solution
```  (434.7 ng/μL)(V1) = (1 ng/μL)(1000 μL)