User:Melissa Novy/Notebook/CHEM-571/2012/10/31

From OpenWetWare
< User:Melissa Novy‎ | Notebook‎ | CHEM-571‎ | 2012‎ | 10
Revision as of 12:40, 6 November 2012 by Melissa Novy (talk | contribs) (Au-Lysozyme Solutions)
Jump to: navigation, search

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Melissa Novy/Notebook/CHEM-571/2012/10/31</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>


  • Make solutions of lysozyme and HAuCl4 at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.

Au-Lysozyme Solutions

  • A 10501 μM solution of HAuCl4 in distilled H2O, made on 2012/09/05 was obtained.
  • 5 mL of 70 μM solution of lysozyme in distilled H2O was made.
    • Add 0.005 g of lysozyme to 5 mL of distilled H2O.
  (70×10-6 M lysozyme)(0.005 L)=3.5×10-7 mol lysozyme
  (3.5×10-7 mol)(14300 g/mol)=0.005005 g lysozyme

  • Please refer to Dhea Patel's entry for calculations, actual molarity, and exact volumes of water, HAuCl4, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.
  • The Au-lysozyme solutions were wrapped in foil to prevent photodegradation and placed in an oven for 4 hours at 80°C.