Difference between revisions of "User:Melissa Novy/Notebook/CHEM-571/2012/10/31"

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==Objectives==
 
==Objectives==
 
* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.
 
* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.
 +
 +
==Au-Lysozyme Solutions==
 +
* A 10501 μM solution of HAuCl<sub>4</sub> in distilled H<sub>2</sub>O, made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05|2012/09/05]] was obtained.
 +
* 5 mL of 70 μM solution of lysozyme in distilled H<sub>2</sub>O was made.
 +
** 0.005 g of lysozyme was added to 5 mL of distilled H<sub>2</sub>O.
 +
CALCULATION
 +
* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31|Dhea Patel's entry]] for calculations and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.
  
  

Revision as of 12:18, 6 November 2012

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Objectives

  • Make solutions of lysozyme and HAuCl4 at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.

Au-Lysozyme Solutions

  • A 10501 μM solution of HAuCl4 in distilled H2O, made on 2012/09/05 was obtained.
  • 5 mL of 70 μM solution of lysozyme in distilled H2O was made.
    • 0.005 g of lysozyme was added to 5 mL of distilled H2O.

CALCULATION

  • Please refer to Dhea Patel's entry for calculations and exact volumes of water, HAuCl4, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.