User:Melissa Novy/Notebook/CHEM-571/2012/09/26

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Objectives

  • Continue optimizing the HRP chemiluminescence assay by adjusting the concentrations of the reactants.
  • Analyze Tris buffers at pH 8 and 10 and at various concentrations with UV-vis spectroscopy.

Spectroscopy Data of Tris Buffers

Graph goes here.

  • Graph of absorbance versus wavelength of Tris buffer in H2O at pH 8 and 10 and at concentrations ranging from 10 μM to 10 mM.