Difference between revisions of "User:Melissa Novy/Notebook/CHEM-571/2012/09/25"

From OpenWetWare
< User:Melissa Novy‎ | Notebook‎ | CHEM-571‎ | 2012‎ | 09
Jump to: navigation, search
(Objectives)
Line 19: Line 19:
 
* Make starter culture media and expression culture media to use with <i>E. coli</i> cells expressing adenosine deaminase enzyme.
 
* Make starter culture media and expression culture media to use with <i>E. coli</i> cells expressing adenosine deaminase enzyme.
 
** No alterations were made to the [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|starter culture media protocol]] or the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media protocol]].
 
** No alterations were made to the [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|starter culture media protocol]] or the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media protocol]].
 +
** Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/25|Keyun Wang's entry]] for actual masses and volumes.
  
 
==Luminol Stock Solution in H<sub>2</sub>O and Carbonate Buffer==
 
==Luminol Stock Solution in H<sub>2</sub>O and Carbonate Buffer==

Revision as of 09:46, 25 September 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Melissa Novy/Notebook/CHEM-571/2012/09/25</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Objectives

Luminol Stock Solution in H2O and Carbonate Buffer

  • Please refer to entry on 2012/09/19 for luminol stock solution protocol and calculations.
    • Actual mass luminol: 0.00443 g
    • Actual concentration luminol: 2.5 mM
    • Actual mass Na2CO3: 0.08 g
    • Actual mass naHCO3: 0.48 g

Horseradish Peroxidase Chemiluminescence Assay

  • Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.