Difference between revisions of "User:Melissa Novy/Notebook/CHEM-571/2012/09/25"

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(Autocreate 2012/09/25 Entry for User:Melissa_Novy/Notebook/CHEM-571)
 
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==Entry title==
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==Objectives==
* Insert content here...
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* Remake 2.5 mM luminol stock solution and 2.3 μL HRP solution to continue HRP chemiluminescence assay.
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* Make starter culture media and expression culture media to use with <i>E. coli</i> cells expressing adenosine deaminase enzyme.
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** No alterations were made to the [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|starter culture media protocol]] or the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media protocol]].
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==Luminol Stock Solution in H<sub>2</sub>O and Carbonate Buffer==
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* Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/19|entry on 2012/09/19]] for luminol stock solution protocol and calculations.
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** Actual mass luminol: 0.00443 g
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** Actual concentration luminol: 2.5 mM
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** Actual mass Na<sub>2</sub>CO<sub>3</sub>: 0.08 g
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** Actual mass naHCO<sub>3</sub>: 0.48 g
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==Horseradish Peroxidase Chemiluminescence Assay==
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* Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.
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Revision as of 09:40, 25 September 2012

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Objectives

  • Remake 2.5 mM luminol stock solution and 2.3 μL HRP solution to continue HRP chemiluminescence assay.
  • Make starter culture media and expression culture media to use with E. coli cells expressing adenosine deaminase enzyme.

Luminol Stock Solution in H2O and Carbonate Buffer

  • Please refer to entry on 2012/09/19 for luminol stock solution protocol and calculations.
    • Actual mass luminol: 0.00443 g
    • Actual concentration luminol: 2.5 mM
    • Actual mass Na2CO3: 0.08 g
    • Actual mass naHCO3: 0.48 g

Horseradish Peroxidase Chemiluminescence Assay

  • Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.