Difference between revisions of "User:Melissa Novy/Notebook/CHEM-571/2012/09/05"

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(Objectives)
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* Prepare serial dilutions of 0.1 M Tris buffer stock solution at the following concentrations: 10 mM, 1 mM, 100 μM, 10 μM.
 
* Prepare serial dilutions of 0.1 M Tris buffer stock solution at the following concentrations: 10 mM, 1 mM, 100 μM, 10 μM.
 
* Remake Au and BSA stock solutions to check for contamination.
 
* Remake Au and BSA stock solutions to check for contamination.
 +
* Resuspend Au/BSA fibers in different concentrations of pH 8 or 10 Tris buffer.
 +
 +
==Au/BSA Fibers==
 +
* Au/BSA solutions were centrifuged at 3000 rpm, 25°C, for 5 min
  
 
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Revision as of 10:07, 5 September 2012

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Objectives

  • Prepare serial dilutions of 0.1 M Tris buffer stock solution at the following concentrations: 10 mM, 1 mM, 100 μM, 10 μM.
  • Remake Au and BSA stock solutions to check for contamination.
  • Resuspend Au/BSA fibers in different concentrations of pH 8 or 10 Tris buffer.

Au/BSA Fibers

  • Au/BSA solutions were centrifuged at 3000 rpm, 25°C, for 5 min