Difference between revisions of "User:Melissa Novy/Notebook/CHEM-571"

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(Gold Nanoparticles)
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==Gold Nanoparticles==
 
==Gold Nanoparticles==
* Research on gold nanoparticles (AuNPs) is being continued from the previous year.  AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy.  Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase or lysozyme rather than BSA.
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* Research on gold nanoparticles (AuNPs) is being continued from the previous year.  AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy and atomic absorption spectroscopy.  Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase (ADA) or lysozyme rather than BSA.
* Protein expression using ADA will be carried out in the near future.
+
* A Bradford assay was used to determine the amount of ADA obtained from protein expressionADA was also mutated with one of three primers and underwent protein expression.
 +
* AuNPs were synthesized with lysozyme at various mole ratios of Au/lysozyme and their presence in solution was analyzed with UV-vis spectroscopy.
 +
 
 +
==Protein Activity Assays==
 +
* Two activity assays of horseradish peroxidase (HRP) were optimized and the activity of HRP was calculated from the data.  The first activity assay used aminoantipyrine (AAP) as the substrate and the reaction was monitored with UV-vis spectroscopy.  The second activity assay monitored the fluorescence of luminol in the presence of HRP.
  
 
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Revision as of 13:27, 12 November 2012

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Gold Nanoparticles

  • Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H2O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy and atomic absorption spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase (ADA) or lysozyme rather than BSA.
  • A Bradford assay was used to determine the amount of ADA obtained from protein expression. ADA was also mutated with one of three primers and underwent protein expression.
  • AuNPs were synthesized with lysozyme at various mole ratios of Au/lysozyme and their presence in solution was analyzed with UV-vis spectroscopy.

Protein Activity Assays

  • Two activity assays of horseradish peroxidase (HRP) were optimized and the activity of HRP was calculated from the data. The first activity assay used aminoantipyrine (AAP) as the substrate and the reaction was monitored with UV-vis spectroscopy. The second activity assay monitored the fluorescence of luminol in the presence of HRP.
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