User:Megan L. Channell/Notebook/Horseradish/2013/10/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span> | |||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |||
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==Objective== | |||
To monitor the kinetics and yield of the HRP-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. | |||
From [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/10/01 | Dr. Hartings]] | |||
==Procedure== | |||
'''1. UV-Vis Absorbance of Reactants, Catalysts, and Products''' | |||
# Take UV-Vis of horseradish peroxidase stock solution. | |||
# Take UV-Vis of luminol of stock solution. The absorbance too high, so the stock solution was diluted 200x. | |||
# Take UV-Vis of 3-aminophthalic acid. | |||
:* Data not taken. | |||
==Figures== | |||
[[Image:HRP-luminol-H2O2-UVVIS.jpg|650 px|]] | |||
==Notes== | |||
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Revision as of 12:28, 10 October 2013
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ObjectiveTo monitor the kinetics and yield of the HRP-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. From Dr. Hartings
Procedure1. UV-Vis Absorbance of Reactants, Catalysts, and Products
FiguresNotes |