Difference between revisions of "User:Megan L. Channell/Notebook/Horseradish/2013/09/25"

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Objective==
 
==Objective==
  
Run SDS-PAGE on the amount of hemoglobin from [[
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Run SDS-PAGE on the amount of hemoglobin from [[User:Megan L. Channell/Notebook/Horseradish/2013/09/24 | yesterday's lab]].
 +
 
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==Protocol==
 +
The procedure came from Dr. Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 | lab notebook]].  Two additional samples were made using the 2 nM of pepsin and  0.2μM of pepstatin solutions yesterday.  It was prepared just like the SDS-PAGE samples except stored in a refrigerator.
 +
 
 +
==Data==
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Observations of the SDS-PAGE with the samples from yesterday.
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*
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[[Image:9 25 2013 cmj SDSPAGE gel.png]]
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*For the wells going left to right:
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# pepstatin 1
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#pepsin 1
 +
#pepsin 2
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#pepstatin 4
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#hemoglobin
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#pepstatin 3
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#pepsin 3
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#pepsin 4
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#pepstatin 2
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#pepsin stock
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#pepstatin stock
  
 
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Latest revision as of 22:24, 26 September 2017

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Objective

Run SDS-PAGE on the amount of hemoglobin from yesterday's lab.

Protocol

The procedure came from Dr. Hartings lab notebook. Two additional samples were made using the 2 nM of pepsin and 0.2μM of pepstatin solutions yesterday. It was prepared just like the SDS-PAGE samples except stored in a refrigerator.

Data

Observations of the SDS-PAGE with the samples from yesterday.

9 25 2013 cmj SDSPAGE gel.png

  • For the wells going left to right:
  1. pepstatin 1
  2. pepsin 1
  3. pepsin 2
  4. pepstatin 4
  5. hemoglobin
  6. pepstatin 3
  7. pepsin 3
  8. pepsin 4
  9. pepstatin 2
  10. pepsin stock
  11. pepstatin stock