User:Megan L. Channell/Notebook/Horseradish/2013/09/24: Difference between revisions
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==Protocol== | ==Protocol== | ||
The procedure for the lab can be found in Dr. Harting's [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 | lab notebook]]. | The procedure for the lab can be found in Dr. Harting's [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 | lab notebook]]. | ||
*Noted differences: | |||
For the concnetration of pepstatin added, the group decided upon 0.2 μM. | **Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid. | ||
The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin) | **For the concnetration of pepstatin added, the group decided upon 0.2 μM. | ||
For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL. | **The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin) | ||
**For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL. | |||
Revision as of 17:41, 30 September 2013
 Biomaterials Design Lab
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ObjectiveUse pepsin to cleve the peptide bonds in hemoglobin and observe its catalytic activity with and without pepstatin. The data collected will be used in a future experiment. ProtocolThe procedure for the lab can be found in Dr. Harting's lab notebook.
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