Difference between revisions of "User:Megan L. Channell/Notebook/Horseradish/2013/09/24"

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(Protocol)
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==Protocol==
 
==Protocol==
 
The procedure for the lab can be found in Dr. Harting's [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 | lab notebook]].  
 
The procedure for the lab can be found in Dr. Harting's [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 | lab notebook]].  
One of noted difference is instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.   
+
*Noted differences:
For the concnetration of pepstatin added, the group decided upon 0.2 μM.
+
**Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.   
The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
+
**For the concnetration of pepstatin added, the group decided upon 0.2 μM.
For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.
+
**The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
 +
**For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.
  
  

Revision as of 16:41, 30 September 2013

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Objective

Use pepsin to cleve the peptide bonds in hemoglobin and observe its catalytic activity with and without pepstatin. The data collected will be used in a future experiment.

Protocol

The procedure for the lab can be found in Dr. Harting's lab notebook.

  • Noted differences:
    • Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.
    • For the concnetration of pepstatin added, the group decided upon 0.2 μM.
    • The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
    • For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.