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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Protocol==
 
==Protocol==
The procedure for the lab can be found in Dr. Harting's [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 | lab notebook]].  
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The procedure for the lab can be found in Dr. Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 | lab notebook]].  
One of noted difference is instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.   
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*Noted differences:
For the concnetration of pepstatin added, the group decided upon 0.2 μM.
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**Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.   
The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
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**For the concnetration of pepstatin added, the group decided upon 0.2 μM.
For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.
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**The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
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**For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.
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==Data==
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* UV-VIS spectra data, taken at 1/2 hour intervals
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[[Image:9.24.13 cmj UVVIS pepsinpepstatin.png|800 px|]]
  
  

Latest revision as of 23:23, 26 September 2017

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Objective

Use pepsin to cleve the peptide bonds in hemoglobin and observe its catalytic activity with and without pepstatin. The data collected will be used in a future experiment.

Protocol

The procedure for the lab can be found in Dr. Hartings lab notebook.

  • Noted differences:
    • Instead of 1.7 M perchloric acid, it was 1 M of perchloric acid.
    • For the concnetration of pepstatin added, the group decided upon 0.2 μM.
    • The stock solution of pepsin was diluted from 12μM to 2nM using .83μL in 5mL total solution (solution being hemoglobin)
    • For a reference in the UV-Vis, hemoglobin stock solution was diluted from 10μL to 1mL.

Data

  • UV-VIS spectra data, taken at 1/2 hour intervals

9.24.13 cmj UVVIS pepsinpepstatin.png