User:Megan L. Channell/Notebook/Horseradish/2013/09/04

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Adenosine and Inosine UV-Vis and Analysis

Objective

  • Collect the data on the UV-Vis from the ionisine dilutions

The inosine samples came from yesterday's protocol

  • Calculate calibration curves for Adenosine and Inosine
  • Run a data regressions on the class' data

Protocol

Yesterday, our adenosine dilutions were much lower than the class' average, so we redid the adenosine samples. A new stock solutions was made using yesterday's protocol and the stock solutions was made from .0809g of adenosine. Adenosine2 9 4 2013 cmj.jpg

Data

A UV-Vis spectra of the new adenosine samples and the inosine samples were collected. The parameters for the UV-Vis were measured between 450 and 200nm. The peak for the insonie spectra was at 249nm. This wavelength was used when calculating the calibration curve. Inosine 9 4 2013 cmj UVVIS.jpg