Difference between revisions of "User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/18"

From OpenWetWare
Jump to: navigation, search
(Results)
(Results)
Line 20: Line 20:
 
==Results==
 
==Results==
 
*Brute force
 
*Brute force
[[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]]  
+
**[[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]]  
[[Image:Bforce_myo.jpg|300px]]
+
**[[Image:Bforce_myo.jpg|300px]]
[[Image:Bforce_myo_10x3.jpg|300px]]   
+
**[[Image:Bforce_myo_10x3.jpg|300px]]   
[[Image:Bforce_myo3.jpg|300px]]
+
**[[Image:Bforce_myo3.jpg|300px]]
 
*Scaffolds after 1 hr in Tris-polydopamine
 
*Scaffolds after 1 hr in Tris-polydopamine
 
[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]]
 
[[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]]

Revision as of 13:48, 20 February 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  • Make polydopamine for nanofibers
  • Myoglobin for procedure

Procedure

  • Preparing scaffolds
    • Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
      • First two scaffolds immersed in solution pH 8.51
      • Second two scaffolds immersed in solution pH 8.59
    • Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
    • Rinse with deionized water and allow scaffold to dry overnight

Results

  • Brute force
    • Photo Feb 18, 1 45 09 PM.jpg
    • Bforce myo.jpg
    • Bforce myo 10x3.jpg
    • Bforce myo3.jpg
  • Scaffolds after 1 hr in Tris-polydopamine

Photo Feb 18, 2 08 38 PM.jpg

  • Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution