Difference between revisions of "User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/02/18"
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==Entry title== | ==Entry title== | ||
| − | * | + | * Make polydopamine for nanofibers |
| + | *Myoglobin for procedure | ||
| + | |||
| + | ==Procedure== | ||
| + | *Preparing scaffolds | ||
| + | **Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour | ||
| + | ***First two scaffolds immersed in solution pH 8.51 | ||
| + | ***Second two scaffolds immersed in solution pH 8.59 | ||
| + | **Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours | ||
| + | **Rinse with deionized water and allow scaffold to dry overnight | ||
| + | |||
| + | ==Results== | ||
| + | *Brute force | ||
| + | [[Image:Photo_Feb_18,_1_45_09_PM.jpg|300px]] | ||
| + | [[Image:Bforce_myo.jpg|300px]]<br.> | ||
| + | [[Image:Bforce_myo_10x3.jpg|300px]] | ||
| + | [[Image:Bforce_myo3.jpg|300px]]<br.> | ||
| + | *Scaffolds after 1 hr in Tris-polydopamine | ||
| + | [[Image:Photo_Feb_18,_2_08_38_PM.jpg|300px]] | ||
| + | *Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution | ||
Revision as of 13:47, 20 February 2014
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