# Difference between revisions of "User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/01/29"

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## Method

• There were a total of 12 gold: nanofiber solutions made, with all of them being the same
• The ratio of myoglobin to gold was 100:1 and the concentration of gold was 0.25mM
• Three of the solutions will have the scaffolds in them as this is our first method
• The other 9 will be used to make the other two methods
• They were put in an oven for 4hrs at 80°C

## Calculations

• Au stock solution:
• 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
• Myoglobin stock solution:
• 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
• Volumes of Au, Myoglobin, and water for each test tube:
• Final [Au]=0.25mM, 5mL total volume

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