Difference between revisions of "User:Megan E. Garber/Notebook/BioE140L lab notebook/2013/04/19"

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(Autocreate 2013/04/19 Entry for User:Megan_E._Garber/Notebook/BioE140L_lab_notebook)
 
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==Entry title==
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==April 13th 2013==
* Insert content here...
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Golden Gate Protocol
 +
*6.5ul ddH2O
 +
*1ul Ligase buffer
 +
* 0.5ul ligase
 +
* 0.5 BsmB1
 +
*1ul of synthon mix (1ul of each synhon vortexted)
 +
* 0.5 ul vector
  
  

Revision as of 12:32, 19 April 2013

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April 13th 2013

Golden Gate Protocol

  • 6.5ul ddH2O
  • 1ul Ligase buffer
  • 0.5ul ligase
  • 0.5 BsmB1
  • 1ul of synthon mix (1ul of each synhon vortexted)
  • 0.5 ul vector