User:Megan E. Garber/Notebook/BioE140L lab notebook/2013/04/19

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April 13th 2013

Golden Gate Protocol

  • 6.5ul ddH2O
  • 1ul Ligase buffer
  • 0.5ul ligase
  • 0.5 BsmB1
  • 1ul of synthon mix (1ul of each synhon vortexted)
  • 0.5 ul vector