Difference between revisions of "User:Megan E. Garber/Notebook/BioE140L lab notebook/2013/04/04"

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(Entry title)
(April 4th 2013)
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==April 4th 2013==
 
==April 4th 2013==
Ligations
+
'''Ligations'''
 
*6.5ul water
 
*6.5ul water
 
*1 ul Ligation Buffer
 
*1 ul Ligation Buffer
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*0.5 ul Ligase
 
*0.5 ul Ligase
  
Transormations
+
'''Transformations'''
 
*1 tube cells
 
*1 tube cells
 
*30 ul KCM
 
*30 ul KCM

Revision as of 09:09, 4 April 2013

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April 4th 2013

Ligations

  • 6.5ul water
  • 1 ul Ligation Buffer
  • 1 ul vector
  • 1ul insert
  • 0.5 ul Ligase

Transformations

  • 1 tube cells
  • 30 ul KCM
  • no water