Difference between revisions of "User:Maximilian Peters"

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(Solutions and Media)
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RPMI
  
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''CHEMICAL NAME'''
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| align="center" style="background:#f0f0f0;"|'''Grams per Liter'''
 +
|-
 +
| ||
 +
|-
 +
| CALCIUM NITRATE Ca(NO3)2 . 4H2O||0,10
 +
|-
 +
| MAGNESIUM SULFATE ANHYDROUS  MgSO4||0,05
 +
|-
 +
| POTASSIUM CHLORIDE  KCl||0,40
 +
|-
 +
| SODIUM CHLORIDE  NaCl||6,00
 +
|-
 +
| SODIUM PHOSPHATE DIBASIC  Na2HPO4||0,80
 +
|-
 +
| ||
 +
|-
 +
| L-ARGININE  ||0,20
 +
|-
 +
| L-ASPARAGINE||0,05
 +
|-
 +
| L-ASPARTIC ACID||0,020
 +
|-
 +
| L-CYSTINE 2HCl||0,07
 +
|-
 +
| L-GLUTAMIC ACID||0,02
 +
|-
 +
| GLYCINE||0,01
 +
|-
 +
| L-HISTIDINE||0,02
 +
|-
 +
| HYDROXY-L-PROLINE  ||0,020
 +
|-
 +
| L-ISOLEUCINE||0,05
 +
|-
 +
| L-LEUCINE||0,05
 +
|-
 +
| L-LYSINE  HCL||0,04
 +
|-
 +
| L-METHIONINE||0,02
 +
|-
 +
| L-PHENYLALANINE||0,02
 +
|-
 +
| L-PROLINE  ||0,02
 +
|-
 +
| L-SERINE  ||0,03
 +
|-
 +
| L-THREONINE||0,02
 +
|-
 +
| L-TRYPTOPHAN||0,01
 +
|-
 +
| L-TYROSINE,  DISODIUM SALT, DIHYDRATE||0,03
 +
|-
 +
| L-VALINE||0,02
 +
|-
 +
| ||
 +
|-
 +
| D-BIOTIN  ||0
 +
|-
 +
| CHOLINE CHLORIDE||0
 +
|-
 +
| FOLIC ACID||0
 +
|-
 +
| myo-INOSITOL||0,04
 +
|-
 +
| NIACINAMIDE  ||0
 +
|-
 +
| P-AMINOBENZOIC ACID  ||0
 +
|-
 +
| D-CALCIUM PANTOTHENATE||0
 +
|-
 +
| PYRIDOXINE  HCL||0
 +
|-
 +
| RIBOFLAVIN||0,0002
 +
|-
 +
| THIAMINE  HCL||0
 +
|-
 +
| CYANOCOBALAMIN  VITAMIN  B12||0
 +
|-
 +
| ||
 +
|-
 +
| DEXTROSE C6H1206||2,00
 +
|-
 +
| GLUTATHIONE  (REDUCED)||0
 +
|-
 +
| PHENOL RED Na||0,01
 +
|-
 +
|
 +
|}
  
 
Reference: http://www.cellsoup.com/cmandrfr.html
 
Reference: http://www.cellsoup.com/cmandrfr.html

Revision as of 05:33, 24 July 2009

Contact Info

Maximilian Peters (an artistic interpretation)

I work in the Your Lab at XYZ University. I learned about OpenWetWare from google, and I've joined because Learn and contribute.

Education

  • Year, PhD, Institute
  • Year, MS, Institute
  • Year, BS, Institute

Research interests

  1. Interest 1
  2. Interest 2
  3. Interest 3

Publications

  1. Kogan NM, Schlesinger M, Peters M, Marincheva G, Beeri R, and Mechoulam R. A cannabinoid anticancer quinone, HU-331, is more potent and less cardiotoxic than doxorubicin: a comparative in vivo study. J Pharmacol Exp Ther. 2007 Aug;322(2):646-53. DOI:10.1124/jpet.107.120865 | PubMed ID:17478614 | HubMed [Paper1]
  2. Mechoulam R, Peters M, Murillo-Rodriguez E, and Hanus LO. Cannabidiol--recent advances. Chem Biodivers. 2007 Aug;4(8):1678-92. DOI:10.1002/cbdv.200790147 | PubMed ID:17712814 | HubMed [Paper2]
  3. Peters M and Kogan NM. HU-331: a cannabinoid quinone, with uncommon cytotoxic properties and low toxicity. Expert Opin Investig Drugs. 2007 Sep;16(9):1405-13. DOI:10.1517/13543784.16.9.1405 | PubMed ID:17714026 | HubMed [Paper3]
  4. Parnas M, Peters M, Dadon D, Lev S, Vertkin I, Slutsky I, and Minke B. Carvacrol is a novel inhibitor of Drosophila TRPL and mammalian TRPM7 channels. Cell Calcium. 2009 Mar;45(3):300-9. DOI:10.1016/j.ceca.2008.11.009 | PubMed ID:19135721 | HubMed [Paper4]
  5. Parnas M, Peters M, and Minke B. Linoleic acid inhibits TRP channels with intrinsic voltage sensitivity: Implications on the mechanism of linoleic acid action. Channels (Austin). 2009 May-Jun;3(3):164-6. PubMed ID:19535910 | HubMed [Paper5]
All Medline abstracts: PubMed | HubMed

Useful links


Solutions and Media

Schneiders S2 medium:

Inorganic salts [g/l]
CaCl2 0.6
MgSO4 (anhyd) 1.807221
KCl 01.06.09
KH2PO4 0.45
NaHCO3 0.4
NaCl 02.01.09
Na2HPO4 0.7
Amino acids [g/l]
b-Alanine 0.5
L-Arginine 0.6
L-Aspartic acid 0.4
L-Cysteine 0.06
L-Cystine • 2HCl 0.026732
L-Glutamic acid 0.8
L-Glutamine 01.08.09
Glycine 0.25
L-Histidine 0.4
L-Isoleucine 0.15
L-Leucine 0.15
L-Lysine 01.01.65
L-Methionine 0.15
L-Proline 01.07.09
L-Serine 0.25
L-Threonine 0.35
L-Tryptophan 0.1
L-Tyrosine 2Na 2H2O 0.720199
L-Valine 0.3
Sugars
D(+)-Glucose 2
D-(+)-Trehalose 2
Other
Fumaric acid 0.06
a-Ketoglutaric acid acid
L(-)-Malic acid 0.6
Succinic acid 0.06
Yeast extract 2

Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf

DMEM - Dulbecco's Modified Eagle's Medium

With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine

CHEMICAL NAME Grams per Liter
CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20 0,26
FERRIC NITRATE 9-HYDRATE Fe(NO3)3. 9H2O 0
MAGNESIUM SULFATE ANHYDROUS MgSO4 0,1
POTASSIUM CHLORIDE KCl 0,40
SODIUM BICARBONATE NaHCO3 3,70
SODIUM CHLORIDE NaCl 6,40
SODIUM PHOSPHATE DIBASIC Na2HPO4 0,11
L-ARGININE, HCL 0,08
L-CYSTINE 2HCl 0,06
GLYCINE 0,030
L-HISTIDINE-HCL-H20 0,04
L-ISOLEUCINE 0,11
L-LEUCINE 0,11
L-LYSINE HCL 0,15
L-METHIONINE 0,030
L-PHENYLALANINE 0,07
L-SERINE 0,04
L-THREONINE 0,1
L-TRYPTOPHAN 0,02
L-TYROSINE, DISODIUM SALT, DIHYDRATE 0,1
L-VALINE 0,09
CHOLINE CHLORIDE 0
FOLIC ACID 0
myo-INOSITOL 0,01
NIACINAMIDE 0
D-CALCIUM PANTOTHENATE 0
PYRIDOXINE HCL 0
RIBOFLAVIN 0
THIAMINE HCL 0
DEXTROSE C6H1206 1,00
PHENOL RED Na 0,02
SODIUM PYRUVATE 0,11

RPMI

CHEMICAL NAME Grams per Liter
CALCIUM NITRATE Ca(NO3)2 . 4H2O 0,10
MAGNESIUM SULFATE ANHYDROUS MgSO4 0,05
POTASSIUM CHLORIDE KCl 0,40
SODIUM CHLORIDE NaCl 6,00
SODIUM PHOSPHATE DIBASIC Na2HPO4 0,80
L-ARGININE 0,20
L-ASPARAGINE 0,05
L-ASPARTIC ACID 0,020
L-CYSTINE 2HCl 0,07
L-GLUTAMIC ACID 0,02
GLYCINE 0,01
L-HISTIDINE 0,02
HYDROXY-L-PROLINE 0,020
L-ISOLEUCINE 0,05
L-LEUCINE 0,05
L-LYSINE HCL 0,04
L-METHIONINE 0,02
L-PHENYLALANINE 0,02
L-PROLINE 0,02
L-SERINE 0,03
L-THREONINE 0,02
L-TRYPTOPHAN 0,01
L-TYROSINE, DISODIUM SALT, DIHYDRATE 0,03
L-VALINE 0,02
D-BIOTIN 0
CHOLINE CHLORIDE 0
FOLIC ACID 0
myo-INOSITOL 0,04
NIACINAMIDE 0
P-AMINOBENZOIC ACID 0
D-CALCIUM PANTOTHENATE 0
PYRIDOXINE HCL 0
RIBOFLAVIN 0,0002
THIAMINE HCL 0
CYANOCOBALAMIN VITAMIN B12 0
DEXTROSE C6H1206 2,00
GLUTATHIONE (REDUCED) 0
PHENOL RED Na 0,01

Reference: http://www.cellsoup.com/cmandrfr.html