Difference between revisions of "User:Maximilian Peters"

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*[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]]
 
*[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]]
  
I learned about [[OpenWetWare]] from google, and I've joined because Learn and contribute.
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I learned about [[OpenWetWare]] from google, and I've joined because I want to learn and contribute.
==Protocols==
 
  
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==Site directed mutagenesis protocol==
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http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis
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==Preparing poly-lysine coated coverslips==
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http://openwetware.org/wiki/User:Maximilian_Peters/polylysine
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==Pharmacology of TRP channels==
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[[User:Maximilian_Peters/TRPharmacalogy|List of antagonists and agonists of TRP channels]]
 
==Useful Links==
 
==Useful Links==
 
===Protein-Protein interaction prediction servers===
 
===Protein-Protein interaction prediction servers===
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==Good enyzmes for cutting PCR products close to the end==
 
==Good enyzmes for cutting PCR products close to the end==
AflII: CCACATGTGG  
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AflIII: CCACATGTGG <br>
AscI: GGCGCGCC  
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AscI: GGCGCGCC <br>
AvaI: CCCCCGGGGG  
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AvaI: CCCCCGGGGG <br>
BamHI: CGGGATCCCG  
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BamHI: CGGGATCCCG <br>
ClaI: CCATCGATGG  
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ClaI: CCATCGATGG <br>
EcoRI: GGAATTCC  
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EcoRI: GGAATTCC <br>
HaeIII: GGGGCCCC  
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HaeIII: GGGGCCCC <br>
KpnI: GGGGTACCCC  
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KpnI: GGGGTACCCC <br>
StuI: AAGGCCTT  
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StuI: AAGGCCTT <br>
XbaI: GCTCTAGAGC  
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XbaI: GCTCTAGAGC <br>
  
Criteria: >90% digestion in 2h, short sequence
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Criteria: >90% digestion in 2h, short sequence<br>
 
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp
 
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp
  
 
==Solutions and Media==
 
==Solutions and Media==
 
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[[User:Maximilian_Peters/Stock_solutions|List of different stock solutions]]<br><br><br>
 
Schneiders S2 medium:
 
Schneiders S2 medium:
  

Latest revision as of 05:24, 18 July 2012

Contact Info

Maximilian Peters (an artistic interpretation)

I learned about OpenWetWare from google, and I've joined because I want to learn and contribute.


Site directed mutagenesis protocol

http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis

Preparing poly-lysine coated coverslips

http://openwetware.org/wiki/User:Maximilian_Peters/polylysine

Pharmacology of TRP channels

List of antagonists and agonists of TRP channels

Useful Links

Protein-Protein interaction prediction servers

http://www.compbio.dundee.ac.uk/www-pips/
http://crdd.osdd.net:8081/ProPrint/submit.jsp
http://www.russell.embl.de/cgi-bin/tools/interprets.pl

Publications

  1. Kogan NM, Schlesinger M, Peters M, Marincheva G, Beeri R, and Mechoulam R. A cannabinoid anticancer quinone, HU-331, is more potent and less cardiotoxic than doxorubicin: a comparative in vivo study. J Pharmacol Exp Ther. 2007 Aug;322(2):646-53. DOI:10.1124/jpet.107.120865 | PubMed ID:17478614 | HubMed [Paper1]
  2. Mechoulam R, Peters M, Murillo-Rodriguez E, and Hanus LO. Cannabidiol--recent advances. Chem Biodivers. 2007 Aug;4(8):1678-92. DOI:10.1002/cbdv.200790147 | PubMed ID:17712814 | HubMed [Paper2]
  3. Peters M and Kogan NM. HU-331: a cannabinoid quinone, with uncommon cytotoxic properties and low toxicity. Expert Opin Investig Drugs. 2007 Sep;16(9):1405-13. DOI:10.1517/13543784.16.9.1405 | PubMed ID:17714026 | HubMed [Paper3]
  4. Parnas M, Peters M, Dadon D, Lev S, Vertkin I, Slutsky I, and Minke B. Carvacrol is a novel inhibitor of Drosophila TRPL and mammalian TRPM7 channels. Cell Calcium. 2009 Mar;45(3):300-9. DOI:10.1016/j.ceca.2008.11.009 | PubMed ID:19135721 | HubMed [Paper4]
  5. Parnas M, Peters M, and Minke B. Linoleic acid inhibits TRP channels with intrinsic voltage sensitivity: Implications on the mechanism of linoleic acid action. Channels (Austin). 2009 May-Jun;3(3):164-6. PubMed ID:19535910 | HubMed [Paper5]
All Medline abstracts: PubMed | HubMed

Useful links

Good enyzmes for cutting PCR products close to the end

AflIII: CCACATGTGG
AscI: GGCGCGCC
AvaI: CCCCCGGGGG
BamHI: CGGGATCCCG
ClaI: CCATCGATGG
EcoRI: GGAATTCC
HaeIII: GGGGCCCC
KpnI: GGGGTACCCC
StuI: AAGGCCTT
XbaI: GCTCTAGAGC

Criteria: >90% digestion in 2h, short sequence
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp

Solutions and Media

List of different stock solutions


Schneiders S2 medium:

Inorganic salts [g/l]
CaCl2 0.6
MgSO4 (anhyd) 1.807221
KCl 01.06.09
KH2PO4 0.45
NaHCO3 0.4
NaCl 02.01.09
Na2HPO4 0.7
Amino acids [g/l]
b-Alanine 0.5
L-Arginine 0.6
L-Aspartic acid 0.4
L-Cysteine 0.06
L-Cystine • 2HCl 0.026732
L-Glutamic acid 0.8
L-Glutamine 01.08.09
Glycine 0.25
L-Histidine 0.4
L-Isoleucine 0.15
L-Leucine 0.15
L-Lysine 01.01.65
L-Methionine 0.15
L-Proline 01.07.09
L-Serine 0.25
L-Threonine 0.35
L-Tryptophan 0.1
L-Tyrosine 2Na 2H2O 0.720199
L-Valine 0.3
Sugars
D(+)-Glucose 2
D-(+)-Trehalose 2
Other
Fumaric acid 0.06
a-Ketoglutaric acid acid
L(-)-Malic acid 0.6
Succinic acid 0.06
Yeast extract 2

Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf

DMEM - Dulbecco's Modified Eagle's Medium

With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine

CHEMICAL NAME Grams per Liter
CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20 0.26
FERRIC NITRATE 9-HYDRATE Fe(NO3)3. 9H2O 0
MAGNESIUM SULFATE ANHYDROUS MgSO4 0.1
POTASSIUM CHLORIDE KCl 0.40
SODIUM BICARBONATE NaHCO3 3.70
SODIUM CHLORIDE NaCl 6.40
SODIUM PHOSPHATE DIBASIC Na2HPO4 0.11
L-ARGININE, HCl 0.08
L-CYSTINE 2HCl 0.06
GLYCINE 0.030
L-HISTIDINE-HCL-H20 0.04
L-ISOLEUCINE 0.11
L-LEUCINE 0.11
L-LYSINE HCL 0.15
L-METHIONINE 0.030
L-PHENYLALANINE 0.07
L-SERINE 0.04
L-THREONINE 0.1
L-TRYPTOPHAN 0.02
L-TYROSINE, DISODIUM SALT, DIHYDRATE 0.1
L-VALINE 0.09
CHOLINE CHLORIDE 0
FOLIC ACID 0
myo-INOSITOL 0.01
NIACINAMIDE 0
D-CALCIUM PANTOTHENATE 0
PYRIDOXINE HCL 0
RIBOFLAVIN 0
THIAMINE HCL 0
DEXTROSE C6H1206 1.00
PHENOL RED Na 0.02
SODIUM PYRUVATE 0.11

Reference: http://www.cellsoup.com/cmandrfr.html


RPMI 1640 with Sodium Bicarbonate, without L- Glutamine,


CHEMICAL NAME Grams per Liter
CALCIUM NITRATE Ca(NO3)2 . 4H2O 0.10
MAGNESIUM SULFATE ANHYDROUS MgSO4 0.05
POTASSIUM CHLORIDE KCl 0.40
SODIUM CHLORIDE NaCl 6.00
SODIUM PHOSPHATE DIBASIC Na2HPO4 0.80
L-ARGININE 0.20
L-ASPARAGINE 0.05
L-ASPARTIC ACID 0.020
L-CYSTINE 2HCl 0.07
L-GLUTAMIC ACID 0.02
GLYCINE 0.01
L-HISTIDINE 0.02
HYDROXY-L-PROLINE 0.020
L-ISOLEUCINE 0.05
L-LEUCINE 0.05
L-LYSINE HCL 0.04
L-METHIONINE 0.02
L-PHENYLALANINE 0.02
L-PROLINE 0.02
L-SERINE 0.03
L-THREONINE 0.02
L-TRYPTOPHAN 0.01
L-TYROSINE. DISODIUM SALT, DIHYDRATE 0.03
L-VALINE 0.02
D-BIOTIN 0
CHOLINE CHLORIDE 0
FOLIC ACID 0
myo-INOSITOL 0.04
NIACINAMIDE 0
P-AMINOBENZOIC ACID 0
D-CALCIUM PANTOTHENATE 0
PYRIDOXINE HCL 0
RIBOFLAVIN 0.0002
THIAMINE HCL 0
CYANOCOBALAMIN VITAMIN B12 0
DEXTROSE C6H1206 2.00
GLUTATHIONE (REDUCED) 0
PHENOL RED Na 0.01

Reference: http://www.cellsoup.com/cmandrfr.html