Difference between revisions of "User:Maximilian Peters"

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*Maximilian Peters
 
*Maximilian Peters
 
*Hebrew University of Jerusalem
 
*Hebrew University of Jerusalem
*Address 1
 
*Address 2
 
*City, State, Country etc.
 
 
*[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]]
 
*[[Special:Emailuser/Maximilian Peters|Email me through OpenWetWare]]
  
I work in the [[Your Lab]] at XYZ University.  I learned about [[OpenWetWare]] from google, and I've joined because Learn and contribute.
+
I learned about [[OpenWetWare]] from google, and I've joined because I want to learn and contribute.
  
==Education==
 
<!--Include info about your educational background-->
 
* Year, PhD, Institute
 
* Year, MS, Institute
 
* Year, BS, Institute
 
  
==Research interests==
+
==Site directed mutagenesis protocol==
<!-- Feel free to add brief descriptions to your research interests as well -->
+
http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis
# Interest 1
+
 
# Interest 2
+
==Preparing poly-lysine coated coverslips==
# Interest 3
+
http://openwetware.org/wiki/User:Maximilian_Peters/polylysine
 +
 
 +
==Pharmacology of TRP channels==
 +
[[User:Maximilian_Peters/TRPharmacalogy|List of antagonists and agonists of TRP channels]]
 +
==Useful Links==
 +
===Protein-Protein interaction prediction servers===
 +
http://www.compbio.dundee.ac.uk/www-pips/<br>
 +
http://crdd.osdd.net:8081/ProPrint/submit.jsp<br>
 +
http://www.russell.embl.de/cgi-bin/tools/interprets.pl<br>
  
 
==Publications==
 
==Publications==
Line 37: Line 37:
 
*[[Help|OpenWetWare help pages]]
 
*[[Help|OpenWetWare help pages]]
  
 +
==Good enyzmes for cutting PCR products close to the end==
 +
AflIII: CCACATGTGG <br>
 +
AscI: GGCGCGCC <br>
 +
AvaI: CCCCCGGGGG <br>
 +
BamHI: CGGGATCCCG <br>
 +
ClaI: CCATCGATGG <br>
 +
EcoRI: GGAATTCC <br>
 +
HaeIII: GGGGCCCC <br>
 +
KpnI: GGGGTACCCC <br>
 +
StuI: AAGGCCTT <br>
 +
XbaI: GCTCTAGAGC <br>
 +
 +
Criteria: >90% digestion in 2h, short sequence<br>
 +
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp
  
 
==Solutions and Media==
 
==Solutions and Media==
 
+
[[User:Maximilian_Peters/Stock_solutions|List of different stock solutions]]<br><br><br>
 
Schneiders S2 medium:
 
Schneiders S2 medium:
  
Line 135: Line 149:
 
| ||
 
| ||
 
|-
 
|-
| CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20||0,26
+
| CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20||0.26
 
|-
 
|-
 
| FERRIC NITRATE 9-HYDRATE  Fe(NO3)3. 9H2O||0
 
| FERRIC NITRATE 9-HYDRATE  Fe(NO3)3. 9H2O||0
 
|-
 
|-
| MAGNESIUM SULFATE ANHYDROUS  MgSO4||0,1
+
| MAGNESIUM SULFATE ANHYDROUS  MgSO4||0.1
 
|-
 
|-
| POTASSIUM CHLORIDE  KCl||0,40
+
| POTASSIUM CHLORIDE  KCl||0.40
 
|-
 
|-
| SODIUM BICARBONATE  NaHCO3||3,70
+
| SODIUM BICARBONATE  NaHCO3||3.70
 
|-
 
|-
| SODIUM CHLORIDE  NaCl||6,40
+
| SODIUM CHLORIDE  NaCl||6.40
 
|-
 
|-
| SODIUM PHOSPHATE DIBASIC  Na2HPO4||0,11
+
| SODIUM PHOSPHATE DIBASIC  Na2HPO4||0.11
 
|-
 
|-
 
| ||
 
| ||
 
|-
 
|-
| L-ARGININE, HCL||0,08
+
| L-ARGININE, HCl||0.08
 
|-
 
|-
| L-CYSTINE 2HCl||0,06
+
| L-CYSTINE 2HCl||0.06
 
|-
 
|-
| GLYCINE||0,030
+
| GLYCINE||0.030
 
|-
 
|-
| L-HISTIDINE-HCL-H20||0,04
+
| L-HISTIDINE-HCL-H20||0.04
 
|-
 
|-
| L-ISOLEUCINE||0,11
+
| L-ISOLEUCINE||0.11
 
|-
 
|-
| L-LEUCINE||0,11
+
| L-LEUCINE||0.11
 
|-
 
|-
| L-LYSINE  HCL||0,15
+
| L-LYSINE  HCL||0.15
 
|-
 
|-
| L-METHIONINE||0,030
+
| L-METHIONINE||0.030
 
|-
 
|-
| L-PHENYLALANINE||0,07
+
| L-PHENYLALANINE||0.07
 
|-
 
|-
| L-SERINE  ||0,04
+
| L-SERINE  ||0.04
 
|-
 
|-
| L-THREONINE||0,1
+
| L-THREONINE||0.1
 
|-
 
|-
| L-TRYPTOPHAN||0,02
+
| L-TRYPTOPHAN||0.02
 
|-
 
|-
| L-TYROSINE,  DISODIUM SALT, DIHYDRATE||0,1
+
| L-TYROSINE,  DISODIUM SALT, DIHYDRATE||0.1
 
|-
 
|-
| L-VALINE||0,09
+
| L-VALINE||0.09
 
|-
 
|-
 
| ||
 
| ||
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| FOLIC ACID||0
 
| FOLIC ACID||0
 
|-
 
|-
| myo-INOSITOL||0,01
+
| myo-INOSITOL||0.01
 
|-
 
|-
 
| NIACINAMIDE  ||0
 
| NIACINAMIDE  ||0
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| ||
 
| ||
 
|-
 
|-
| DEXTROSE C6H1206||1,00
+
| DEXTROSE C6H1206||1.00
 
|-
 
|-
| PHENOL RED Na||0,02
+
| PHENOL RED Na||0.02
 
|-
 
|-
| SODIUM PYRUVATE||0,11
+
| SODIUM PYRUVATE||0.11
 
|-
 
|-
 
|  
 
|  
 
|}
 
|}
  
 +
Reference: http://www.cellsoup.com/cmandrfr.html
  
 +
 +
<b>RPMI 1640</b>
 +
with Sodium Bicarbonate, without L- Glutamine,
 +
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''CHEMICAL NAME'''
 +
| align="center" style="background:#f0f0f0;"|'''Grams per Liter'''
 +
|-
 +
| ||
 +
|-
 +
| CALCIUM NITRATE Ca(NO3)2 . 4H2O||0.10
 +
|-
 +
| MAGNESIUM SULFATE ANHYDROUS  MgSO4||0.05
 +
|-
 +
| POTASSIUM CHLORIDE  KCl||0.40
 +
|-
 +
| SODIUM CHLORIDE  NaCl||6.00
 +
|-
 +
| SODIUM PHOSPHATE DIBASIC  Na2HPO4||0.80
 +
|-
 +
| ||
 +
|-
 +
| L-ARGININE  ||0.20
 +
|-
 +
| L-ASPARAGINE||0.05
 +
|-
 +
| L-ASPARTIC ACID||0.020
 +
|-
 +
| L-CYSTINE 2HCl||0.07
 +
|-
 +
| L-GLUTAMIC ACID||0.02
 +
|-
 +
| GLYCINE||0.01
 +
|-
 +
| L-HISTIDINE||0.02
 +
|-
 +
| HYDROXY-L-PROLINE  ||0.020
 +
|-
 +
| L-ISOLEUCINE||0.05
 +
|-
 +
| L-LEUCINE||0.05
 +
|-
 +
| L-LYSINE  HCL||0.04
 +
|-
 +
| L-METHIONINE||0.02
 +
|-
 +
| L-PHENYLALANINE||0.02
 +
|-
 +
| L-PROLINE  ||0.02
 +
|-
 +
| L-SERINE  ||0.03
 +
|-
 +
| L-THREONINE||0.02
 +
|-
 +
| L-TRYPTOPHAN||0.01
 +
|-
 +
| L-TYROSINE.  DISODIUM SALT, DIHYDRATE||0.03
 +
|-
 +
| L-VALINE||0.02
 +
|-
 +
| ||
 +
|-
 +
| D-BIOTIN  ||0
 +
|-
 +
| CHOLINE CHLORIDE||0
 +
|-
 +
| FOLIC ACID||0
 +
|-
 +
| myo-INOSITOL||0.04
 +
|-
 +
| NIACINAMIDE  ||0
 +
|-
 +
| P-AMINOBENZOIC ACID  ||0
 +
|-
 +
| D-CALCIUM PANTOTHENATE||0
 +
|-
 +
| PYRIDOXINE  HCL||0
 +
|-
 +
| RIBOFLAVIN||0.0002
 +
|-
 +
| THIAMINE  HCL||0
 +
|-
 +
| CYANOCOBALAMIN  VITAMIN  B12||0
 +
|-
 +
| ||
 +
|-
 +
| DEXTROSE C6H1206||2.00
 +
|-
 +
| GLUTATHIONE  (REDUCED)||0
 +
|-
 +
| PHENOL RED Na||0.01
 +
|-
 +
|
 +
|}
  
 
Reference: http://www.cellsoup.com/cmandrfr.html
 
Reference: http://www.cellsoup.com/cmandrfr.html

Latest revision as of 06:24, 18 July 2012

Contact Info

Maximilian Peters (an artistic interpretation)

I learned about OpenWetWare from google, and I've joined because I want to learn and contribute.


Site directed mutagenesis protocol

http://openwetware.org/wiki/User:Maximilian_Peters/site-directed-mutagenesis

Preparing poly-lysine coated coverslips

http://openwetware.org/wiki/User:Maximilian_Peters/polylysine

Pharmacology of TRP channels

List of antagonists and agonists of TRP channels

Useful Links

Protein-Protein interaction prediction servers

http://www.compbio.dundee.ac.uk/www-pips/
http://crdd.osdd.net:8081/ProPrint/submit.jsp
http://www.russell.embl.de/cgi-bin/tools/interprets.pl

Publications

  1. Kogan NM, Schlesinger M, Peters M, Marincheva G, Beeri R, and Mechoulam R. A cannabinoid anticancer quinone, HU-331, is more potent and less cardiotoxic than doxorubicin: a comparative in vivo study. J Pharmacol Exp Ther. 2007 Aug;322(2):646-53. DOI:10.1124/jpet.107.120865 | PubMed ID:17478614 | HubMed [Paper1]
  2. Mechoulam R, Peters M, Murillo-Rodriguez E, and Hanus LO. Cannabidiol--recent advances. Chem Biodivers. 2007 Aug;4(8):1678-92. DOI:10.1002/cbdv.200790147 | PubMed ID:17712814 | HubMed [Paper2]
  3. Peters M and Kogan NM. HU-331: a cannabinoid quinone, with uncommon cytotoxic properties and low toxicity. Expert Opin Investig Drugs. 2007 Sep;16(9):1405-13. DOI:10.1517/13543784.16.9.1405 | PubMed ID:17714026 | HubMed [Paper3]
  4. Parnas M, Peters M, Dadon D, Lev S, Vertkin I, Slutsky I, and Minke B. Carvacrol is a novel inhibitor of Drosophila TRPL and mammalian TRPM7 channels. Cell Calcium. 2009 Mar;45(3):300-9. DOI:10.1016/j.ceca.2008.11.009 | PubMed ID:19135721 | HubMed [Paper4]
  5. Parnas M, Peters M, and Minke B. Linoleic acid inhibits TRP channels with intrinsic voltage sensitivity: Implications on the mechanism of linoleic acid action. Channels (Austin). 2009 May-Jun;3(3):164-6. PubMed ID:19535910 | HubMed [Paper5]
All Medline abstracts: PubMed | HubMed

Useful links

Good enyzmes for cutting PCR products close to the end

AflIII: CCACATGTGG
AscI: GGCGCGCC
AvaI: CCCCCGGGGG
BamHI: CGGGATCCCG
ClaI: CCATCGATGG
EcoRI: GGAATTCC
HaeIII: GGGGCCCC
KpnI: GGGGTACCCC
StuI: AAGGCCTT
XbaI: GCTCTAGAGC

Criteria: >90% digestion in 2h, short sequence
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp

Solutions and Media

List of different stock solutions


Schneiders S2 medium:

Inorganic salts [g/l]
CaCl2 0.6
MgSO4 (anhyd) 1.807221
KCl 01.06.09
KH2PO4 0.45
NaHCO3 0.4
NaCl 02.01.09
Na2HPO4 0.7
Amino acids [g/l]
b-Alanine 0.5
L-Arginine 0.6
L-Aspartic acid 0.4
L-Cysteine 0.06
L-Cystine • 2HCl 0.026732
L-Glutamic acid 0.8
L-Glutamine 01.08.09
Glycine 0.25
L-Histidine 0.4
L-Isoleucine 0.15
L-Leucine 0.15
L-Lysine 01.01.65
L-Methionine 0.15
L-Proline 01.07.09
L-Serine 0.25
L-Threonine 0.35
L-Tryptophan 0.1
L-Tyrosine 2Na 2H2O 0.720199
L-Valine 0.3
Sugars
D(+)-Glucose 2
D-(+)-Trehalose 2
Other
Fumaric acid 0.06
a-Ketoglutaric acid acid
L(-)-Malic acid 0.6
Succinic acid 0.06
Yeast extract 2

Reference: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/schneiders_insect_media.Par.0001.File.tmp/schneiders_insect_media.pdf

DMEM - Dulbecco's Modified Eagle's Medium

With 1000 mg/l glucose, and Sodium Bicarbonate, without Glutamine

CHEMICAL NAME Grams per Liter
CALCIUM CHLORIDE DIHYDRATE CaCl2. 2H20 0.26
FERRIC NITRATE 9-HYDRATE Fe(NO3)3. 9H2O 0
MAGNESIUM SULFATE ANHYDROUS MgSO4 0.1
POTASSIUM CHLORIDE KCl 0.40
SODIUM BICARBONATE NaHCO3 3.70
SODIUM CHLORIDE NaCl 6.40
SODIUM PHOSPHATE DIBASIC Na2HPO4 0.11
L-ARGININE, HCl 0.08
L-CYSTINE 2HCl 0.06
GLYCINE 0.030
L-HISTIDINE-HCL-H20 0.04
L-ISOLEUCINE 0.11
L-LEUCINE 0.11
L-LYSINE HCL 0.15
L-METHIONINE 0.030
L-PHENYLALANINE 0.07
L-SERINE 0.04
L-THREONINE 0.1
L-TRYPTOPHAN 0.02
L-TYROSINE, DISODIUM SALT, DIHYDRATE 0.1
L-VALINE 0.09
CHOLINE CHLORIDE 0
FOLIC ACID 0
myo-INOSITOL 0.01
NIACINAMIDE 0
D-CALCIUM PANTOTHENATE 0
PYRIDOXINE HCL 0
RIBOFLAVIN 0
THIAMINE HCL 0
DEXTROSE C6H1206 1.00
PHENOL RED Na 0.02
SODIUM PYRUVATE 0.11

Reference: http://www.cellsoup.com/cmandrfr.html


RPMI 1640 with Sodium Bicarbonate, without L- Glutamine,


CHEMICAL NAME Grams per Liter
CALCIUM NITRATE Ca(NO3)2 . 4H2O 0.10
MAGNESIUM SULFATE ANHYDROUS MgSO4 0.05
POTASSIUM CHLORIDE KCl 0.40
SODIUM CHLORIDE NaCl 6.00
SODIUM PHOSPHATE DIBASIC Na2HPO4 0.80
L-ARGININE 0.20
L-ASPARAGINE 0.05
L-ASPARTIC ACID 0.020
L-CYSTINE 2HCl 0.07
L-GLUTAMIC ACID 0.02
GLYCINE 0.01
L-HISTIDINE 0.02
HYDROXY-L-PROLINE 0.020
L-ISOLEUCINE 0.05
L-LEUCINE 0.05
L-LYSINE HCL 0.04
L-METHIONINE 0.02
L-PHENYLALANINE 0.02
L-PROLINE 0.02
L-SERINE 0.03
L-THREONINE 0.02
L-TRYPTOPHAN 0.01
L-TYROSINE. DISODIUM SALT, DIHYDRATE 0.03
L-VALINE 0.02
D-BIOTIN 0
CHOLINE CHLORIDE 0
FOLIC ACID 0
myo-INOSITOL 0.04
NIACINAMIDE 0
P-AMINOBENZOIC ACID 0
D-CALCIUM PANTOTHENATE 0
PYRIDOXINE HCL 0
RIBOFLAVIN 0.0002
THIAMINE HCL 0
CYANOCOBALAMIN VITAMIN B12 0
DEXTROSE C6H1206 2.00
GLUTATHIONE (REDUCED) 0
PHENOL RED Na 0.01

Reference: http://www.cellsoup.com/cmandrfr.html