User:Matthewmeisel

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Revision as of 09:12, 14 June 2006 by Matthewmeisel (talk | contribs) (Wed Jun 14)
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Quick link: iGEM:Harvard/2006

Notebook

Week 1

Wed Jun 14

Transformation of standard components

  • cultured bacteria did grow overnight
  • 1 mL of each tube reserved to make glycerol stock
  • extracted DNA from remaining 4 mL using standard protocol, eluted with water

Tue Jun 13

DNA nanostructure reaction PCR

  • order of lanes: 1) 1kb ladder, 2) full rxn (oligos + scaffold), 3) just scaffold, 4) just oligos
  • run on 2% agarose gel
  • appears that reaction was successful (upload gel image)

Transformation of standard components

Mon Jun 12

DNA nanostructure reaction PCR

Transformation of standard components

Bibliography

  1. Shih WM, Quispe JD, and Joyce GF. A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron. Nature. 2004 Feb 12;427(6975):618-21. DOI:10.1038/nature02307 | PubMed ID:14961116 | HubMed [shih0]
  1. Shih WM and Spudich JA. The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle. J Biol Chem. 2001 Jun 1;276(22):19491-4. DOI:10.1074/jbc.M010887200 | PubMed ID:11278776 | HubMed [shih1]
  1. Shih WM, Gryczynski Z, Lakowicz JR, and Spudich JA. A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell. 2000 Sep 1;102(5):683-94. PubMed ID:11007486 | HubMed [shih2]


Other projects

My work in Biophysics 101

Contact info

Email me: (my last name) at fas dot harvard dot edu