Difference between revisions of "User:Matthewmeisel"

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(Tue Jun 13: nb)
m (Wed Jun 14: nb)
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* cultured bacteria did grow overnight
 
* cultured bacteria did grow overnight
 
* 1 mL of each tube reserved to make glycerol stock
 
* 1 mL of each tube reserved to make glycerol stock
* extracted DNA from remaining 4 mL using [[Miniprep/Qiagen_kit|standard protocol]]
+
* extracted DNA from remaining 4 mL using [[Miniprep/Qiagen_kit|standard protocol]], used water for final extraction
  
 
====Tue Jun 13====
 
====Tue Jun 13====

Revision as of 08:39, 14 June 2006

Quick link: iGEM:Harvard/2006

Notebook

Week 1

Wed Jun 14

Transformation of standard components

  • cultured bacteria did grow overnight
  • 1 mL of each tube reserved to make glycerol stock
  • extracted DNA from remaining 4 mL using standard protocol, used water for final extraction

Tue Jun 13

DNA nanostructure reaction PCR

  • order of lanes: 1) 1kb ladder, 2) full rxn (oligos + scaffold), 3) just scaffold, 4) just oligos
  • run on 2% agarose gel
  • appears that reaction was successful (upload gel image)

Transformation of standard components

Mon Jun 12

DNA nanostructure reaction PCR

  • standard protocol with materials from Shawn

Transformation of standard components

Bibliography

  1. Shih WM, Quispe JD, and Joyce GF. A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron. Nature. 2004 Feb 12;427(6975):618-21. DOI:10.1038/nature02307 | PubMed ID:14961116 | HubMed [shih0]
  1. Shih WM and Spudich JA. The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle. J Biol Chem. 2001 Jun 1;276(22):19491-4. DOI:10.1074/jbc.M010887200 | PubMed ID:11278776 | HubMed [shih1]
  1. Shih WM, Gryczynski Z, Lakowicz JR, and Spudich JA. A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell. 2000 Sep 1;102(5):683-94. PubMed ID:11007486 | HubMed [shih2]


Other projects

My work in Biophysics 101

Contact info

Email me: (my last name) at fas dot harvard dot edu