User:Matt Hartings/Notebook/Photosynthesis/2013/04/04: Difference between revisions
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# Absorbance measurements were calculated using the following equation: A = -log[(SampleSignal-DarkSignal)/(ReferenceSignal-DarkSignal)] | # Absorbance measurements were calculated using the following equation: A = -log[(SampleSignal-DarkSignal)/(ReferenceSignal-DarkSignal)] | ||
==Mb catalysis H2O== | |||
Following the description from [[User:Matt_Hartings/Notebook/Photosynthesis/2013/04/02|Tuesday]]. | |||
# 22.0mg of o-phenylenediamine in 22mL of H2O | |||
# 1.134 ml 30% H2O2 +8.866 ml H2O | |||
# 15.0mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1.5 mL of H2O. | |||
<u>Samples were made by</u> | |||
# 2.025 mL of the OPD solution | |||
# 0.225 mL of the Mb solution | |||
# Start the scan | |||
## 1,000 us integration time | |||
## Average over 500 scans | |||
## Run for 10 minutes (600,000,000 us) | |||
# Add 0.75mL of the H2O2 solution | |||
## Added after scan reached 1% | |||
# Kinetics scans were run at 5, 15, 25, 35, and 45C. Several before and after spectra were also taken, as were the dark and reference signal spectra. | |||
# Absorbance measurements were calculated using the following equation: A = -log[(SampleSignal-DarkSignal)/(ReferenceSignal-DarkSignal)] | |||
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Revision as of 09:16, 4 April 2013
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Mb catalysis DMSOFollowing the description from Tuesday. Stock solutions used
Samples were made by
Mb catalysis H2OFollowing the description from Tuesday.
Samples were made by
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