Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2013/03/18"

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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
 
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Latest revision as of 21:33, 26 September 2017

Hartings AU Photosynthesis Lab Header.png Protein Re-engineering Report.pngMain project page
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Mb in DMSO

Starting with the lyophilized samples that I made on the 11th I am going to make samples in DMSO with crown or (nBu4)NPF6 made on the 12th

  1. Starting makeup of sample lyophilized at pH 3
      1. 0.0115g Mb
      2. 0.2005g D-sorbitol
      3. 0.110g (1450MW) PEG
      4. 10mL 10mM citrate pH 3
    1. (Sample 1) Measured 3.8mg of solid and added 1mL of DMSO with (nBu4)NPF6
    2. (Sample 2) Measured 5.8mg of solid and added 1mL of DMSO with 18-crown-6
  2. Starting makeup of sample lyophilized at pH 5
      1. 0.0110g Mb
      2. 0.1946g D-sorbitol
      3. 0.100g (1450MW) PEG
      4. 10mL 10mM acetate pH 5
    1. (Sample 1) Measured 6.2mg of solid and added 1mL of DMSO with (nBu4)NPF6
    2. (Sample 2) Measured 9.8 mg of solid and added 1mL of DMSO with 18-crown-6
  3. Starting makeup of sample lyophilized at pH 7
      1. 0.020g Mb
      2. 0.1998g D-sorbitol
      3. 0.1085g (1450MW) PEG
      4. 10mL 10mM phosphate pH 7
    1. (Sample 1) Measured 11.6 mg of solid and added 1mL of DMSO with (nBu4)NPF6
    2. (Sample 2) Measured 12.2 mg of solid and added 1mL of DMSO with 18-crown-6
  4. Starting makeup of sample lyophilized at pH 9
      1. 0.0118g Mb
      2. 0.1847g D-sorbitol
      3. 0.106g (1450MW) PEG
      4. 10mL 10mM tris pH 9
    1. (Sample 1) Measured 6.2 mg of solid and added 1mL of DMSO with (nBu4)NPF6
    2. (Sample 2) Measured 7.9 mg of solid and added 1mL of DMSO with 18-crown-6