User:Matt Hartings/Notebook/Photosynthesis/2013/03/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | |style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Mb in DMSO== | ||
Starting with the lyophilized samples that I made on [[User:Matt_Hartings/Notebook/Photosynthesis/2013/03/11|the 11th]] I am going to make samples in DMSO with crown or (nBu<sub>4</sub>)NPF<sub>6</sub> made on [[User:Matt_Hartings/Notebook/Photosynthesis/2013/03/12|the 12th]] | |||
# Starting makeup of sample lyophilized at pH 3 | |||
### 0.0115g Mb | |||
### 0.2005g D-sorbitol | |||
### 0.110g (1450MW) PEG | |||
### 10mL 10mM citrate pH 3 | |||
## (Sample 1) Measured 3.8mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub> | |||
## (Sample 2) Measured 5.8mg of solid and added 1mL of DMSO with 18-crown-6 | |||
# Starting makeup of sample lyophilized at pH 5 | |||
### 0.0110g Mb | |||
### 0.1946g D-sorbitol | |||
### 0.100g (1450MW) PEG | |||
### 10mL 10mM acetate pH 5 | |||
## (Sample 1) Measured 6.2mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub> | |||
## (Sample 2) Measured 9.8 mg of solid and added 1mL of DMSO with 18-crown-6 | |||
# Starting makeup of sample lyophilized at pH 7 | |||
### 0.020g Mb | |||
### 0.1998g D-sorbitol | |||
### 0.1085g (1450MW) PEG | |||
### 10mL 10mM phosphate pH 7 | |||
## (Sample 1) Measured 11.6 mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub> | |||
## (Sample 2) Measured 12.2 mg of solid and added 1mL of DMSO with 18-crown-6 | |||
# Starting makeup of sample lyophilized at pH 9 | |||
### 0.0118g Mb | |||
### 0.1847g D-sorbitol | |||
### 0.106g (1450MW) PEG | |||
### 10mL 10mM tris pH 9 | |||
## (Sample 1) Measured 6.2 mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub> | |||
## (Sample 2) Measured 7.9 mg of solid and added 1mL of DMSO with 18-crown-6 | |||
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Latest revision as of 22:33, 26 September 2017
Protein Re-engineering | Main project page Previous entry Next entry |
Mb in DMSOStarting with the lyophilized samples that I made on the 11th I am going to make samples in DMSO with crown or (nBu4)NPF6 made on the 12th
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