Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2013/03/12"

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==Objective==
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==Solvents for protein stabilization==
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Prepping solvents for looking at protein stability in organic solvents.
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# Acetonitrile
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## 0.4175g (nBu)<sub>4</sub>NPF<sub>6</sub> + 25mL acetonitrile
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## 0.6752g 18-crown-6 + 25mL acetonitrile
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## 0.4033g (nBu)<sub>4</sub>NPF<sub>6</sub> +0.6756g 18-crown-6 + 25mL acetonitrile
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# Hexanes
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## 0.4021g (nBu)<sub>4</sub>NPF<sub>6</sub> + 25mL hexanes
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## 0.6697g 18-crown-6 + 25mL hexanes
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## 0.4012g (nBu)<sub>4</sub>NPF<sub>6</sub> +0.6892g 18-crown-6 + 25mL hexanes
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# DMSO
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## 0.4129g (nBu)<sub>4</sub>NPF<sub>6</sub> + 25mL DMSO
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## 0.6872g 18-crown-6 + 25mL DMSO
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## ran out of (nBu)<sub>4</sub>NPF<sub>6</sub> for this joint solvent with + 18-crown-6
  
  

Revision as of 11:21, 12 March 2013

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Solvents for protein stabilization

Prepping solvents for looking at protein stability in organic solvents.

  1. Acetonitrile
    1. 0.4175g (nBu)4NPF6 + 25mL acetonitrile
    2. 0.6752g 18-crown-6 + 25mL acetonitrile
    3. 0.4033g (nBu)4NPF6 +0.6756g 18-crown-6 + 25mL acetonitrile
  1. Hexanes
    1. 0.4021g (nBu)4NPF6 + 25mL hexanes
    2. 0.6697g 18-crown-6 + 25mL hexanes
    3. 0.4012g (nBu)4NPF6 +0.6892g 18-crown-6 + 25mL hexanes
  1. DMSO
    1. 0.4129g (nBu)4NPF6 + 25mL DMSO
    2. 0.6872g 18-crown-6 + 25mL DMSO
    3. ran out of (nBu)4NPF6 for this joint solvent with + 18-crown-6