Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2013/02/26"

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(Autocreate 2013/02/26 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
 
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==Objective==
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==Protein Dialysis==
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In order to prepare the Mn-Hb samples for purification, I am dialyzing the samples.  
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# Make 4L of 10mM Tris 50mM NaCl, pH 8.5
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## Measured 11.681 g NaCl
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## Measured 4.860 g Tris
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# I split the crude Mn-Hb solution over 2 dialysis tubes (snakeskin).
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# The dialysis tubes were places in the buffer and am storing them in the cold room overnight
  
  

Revision as of 07:23, 26 February 2013

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Protein Dialysis

In order to prepare the Mn-Hb samples for purification, I am dialyzing the samples.

  1. Make 4L of 10mM Tris 50mM NaCl, pH 8.5
    1. Measured 11.681 g NaCl
    2. Measured 4.860 g Tris
  2. I split the crude Mn-Hb solution over 2 dialysis tubes (snakeskin).
  3. The dialysis tubes were places in the buffer and am storing them in the cold room overnight