Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2013/01/31"

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Place in oven at 130C at 9:45am
 
Place in oven at 130C at 9:45am
  
 +
Abigail and I checked the reaction at 12pm, the solution was brown. This is much different than the first time we performed the reaction, where there was a clear solution with a percipitate. We are going to let it run until 1 and then let it cool.
  
 
==Transformation==
 
==Transformation==
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## 100mL water
 
## 100mL water
 
## autoclave
 
## autoclave
 +
## add 100uL of 100mg/ml ampicilin
 +
## Pour plates
 +
#
  
 
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Revision as of 10:11, 31 January 2013

Hartings AU Photosynthesis Lab Header.png Protein Re-engineering <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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MOF

Abigail and I are trying a new MOF synthesis. 51.8 mg DO2A 61.0 mg Zn(NO3)2 6H20 3 mL of diethylformamide

in a scint vial with a teflon cap

sonicate to create a suspension

Place in oven at 130C at 9:45am

Abigail and I checked the reaction at 12pm, the solution was brown. This is much different than the first time we performed the reaction, where there was a clear solution with a percipitate. We are going to let it run until 1 and then let it cool.

Transformation

  1. Make plates
    1. 2.5g LB
    2. 2.0g Agar
    3. 100mL water
    4. autoclave
    5. add 100uL of 100mg/ml ampicilin
    6. Pour plates